von Hippel-Lindau tumor suppressor protein stimulation by thrombin involves RhoA activation

International Journal of Cancer. Journal International Du Cancer
Sandra TurcotteRichard Béliveau

Abstract

Inactivation of the von Hippel-Lindau (VHL) tumor suppressor gene is associated with the development of vascular tumors including renal cell carcinoma. Aside from the role played by the VHL protein (pVHL) in negative regulation of hypoxia-inducible factor, 41F-1alpha, pVHL also takes part in cytoskeletal organization. Thrombin is a serine protease involved in angiogenesis and in cancer progression and its action is mediated by the protease-activated receptors (PARs). In several cell types, thrombin induces reorganization of the cytoskeleton along with RhoA activation. Thus, we conducted an investigation on the capacity of thrombin to regulate pVHL expression. Our results demonstrated that VHL mRNA and protein levels were increased by thrombin in cultured renal cancer cells. Cytoplasmic pVHL was redistributed to perinuclear regions and membrane fractions following thrombin treatments. Stimulation of Caki-1 cells with PAR1, PAR2 and PAR4 agonist peptides demonstrated that PAR1 was the receptor involved in thrombin-induced pVHL expression. Western blot analysis confirmed that these cells express PAR1 and that its expression was increased by thrombin. PAR1 activation by both thrombin and an agonist peptide stimulated renal cancer c...Continue Reading

References

Aug 19, 1991·FEBS Letters·U B RasmussenE Van Obberghen-Schilling
Aug 1, 1995·Nature Medicine·O IliopoulosW G Kaelin
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·S LeeR D Klausner
Dec 31, 1997·Medicine·E R Maher, W G Kaelin
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W F XuD C Foster
Sep 3, 1998·Molecular Biology of the Cell·V Vouret-CraviariE Van Obberghen-Schilling
Sep 24, 1999·Biochemical and Biophysical Research Communications·H OkudaT Shuin
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S R Coughlin
Jun 6, 2000·The Journal of Biological Chemistry·D E RichardJ Pouyssegur
Aug 19, 2000·Circulation Research·G P van Nieuw AmerongenV W van Hinsbergh
Oct 12, 2000·Cellular Signalling·T MatozakiY Takai
Mar 10, 2001·Experimental Cell Research·K Kondo, W G Kaelin
Mar 17, 2001·International Journal of Cancer. Journal International Du Cancer·A R SchoenfeldR D Burk
Apr 21, 2001·Oncogene·I P WhiteheadC J Der
May 16, 2001·Biochemical and Biophysical Research Communications·M ZiegerR Kaufmann
Dec 31, 2002·Journal of the American Society of Nephrology : JASN·Claire C Sharpe, Bruce M Hendry
Jan 3, 2003·Nature Cell Biology·Alexander HergovichWilhelm Krek
Jan 30, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yong-Jun YinRachel Bar-Shavit
May 29, 2003·The Journal of Biological Chemistry·Stephan VogtStefan Offermanns
Jun 13, 2003·Molecular Biology of the Cell·Shigeko YamashiroFumio Matsumura
Oct 30, 2003·American Journal of Physiology. Renal Physiology·Sandra TurcotteRichard Beliveau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.