Jul 29, 2020

VoPo leverages cellular heterogeneity for predictive modeling of single-cell data

Nature Communications
Natalie StanleyNima Aghaeepour

Abstract

High-throughput single-cell analysis technologies produce an abundance of data that is critical for profiling the heterogeneity of cellular systems. We introduce VoPo (https://github.com/stanleyn/VoPo), a machine learning algorithm for predictive modeling and comprehensive visualization of the heterogeneity captured in large single-cell datasets. In three mass cytometry datasets, with the largest measuring hundreds of millions of cells over hundreds of samples, VoPo defines phenotypically and functionally homogeneous cell populations. VoPo further outperforms state-of-the-art machine learning algorithms in classification tasks, and identified immune-correlates of clinically-relevant parameters.

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Mentioned in this Paper

High-Throughput RNA Sequencing
Classification
Profile (Lab Procedure)
Methyl coenzyme M
Cytometry

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