VP4 protein of porcine rotavirus strain OSU expressed by a baculovirus recombinant induces neutralizing antibodies.

Virology
K NishikawaM Gorziglia

Abstract

The complete VP4 gene of porcine rotavirus strain OSU has been inserted into a baculovirus expression vector under the control of the polyhedrin promoter. The VP4 outer capsid protein, which is a major neutralization antigen in rotavirus, was expressed in high yield in Spodoptera frugiperda cells. Reactivity with polyclonal and monoclonal antibodies suggested that neutralizing epitopes were functionally unaltered on the expressed VP4. The VP4 produced in this system also induced antibodies in guinea pigs which inhibited hemagglutination of OSU and neutralized its infectivity to high titer. The available evidence suggests that the VP4 expressed in insect cells maintained its antigenic configuration and may prove useful in elucidation of (1) the extent of VP4 polymorphism among human and animal rotaviruses and (2) the distribution of VP4 among these viruses.

References

Dec 23, 1988·Nucleic Acids Research·K Nishikawa, M Gorziglia
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M GorzigliaR M Chanock
Jan 1, 1983·The Journal of Infectious Diseases·A Z KapikianR M Chanock

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Citations

Jan 1, 1991·Archives of Virology·H S Nagesha, I H Holmes
Jan 1, 1993·Vaccine·R F Bishop
Nov 1, 1993·Veterinary Microbiology·P S Paul, Y S Lyoo
Sep 1, 1990·Baillière's Clinical Gastroenterology·J Flores, A Z Kapikian
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M GorzigliaR M Chanock
Oct 13, 1992·Annals of the New York Academy of Sciences·M J BetenbaughJ J Eiden
Jan 19, 2008·Clinical Microbiology Reviews·Penelope H Dennehy
Oct 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wanqiang LiKangcheng Pan
Sep 1, 1990·Journal of Virology·M GorzigliaR L Ward
Jul 1, 1996·Clinical Microbiology Reviews·K Midthun, A Z Kapikian
Jul 1, 1992·Journal of Virology·M Gorziglia, A Z Kapikian
Nov 1, 1993·Journal of Clinical Microbiology·B Li, M Gorziglia
Jun 1, 1991·Journal of Virology·G LarraldeM Gorziglia
Jan 1, 1993·Journal of Virology·B LiM Gorziglia

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