PMID: 9188633Jul 1, 1997Paper

Vpr of simian immunodeficiency virus of African green monkeys is required for replication in macaque macrophages and lymphocytes

Journal of Virology
B J Campbell, V M Hirsch

Abstract

The genomes of simian immunodeficiency viruses isolated from African green monkeys (SIVagm) contain a single accessory gene homolog of human immunodeficiency virus type 1 (HIV-1) vpr. This genomic organization differs from that of SIVsm-SIVmac-HIV-2 group viruses, which contain two gene homologs, designated vpr and vpx, which in combination appear to share the functions of HIV-1 vpr. The in vitro role of the SIVagm homolog was evaluated with molecularly cloned, pathogenic SIVagm9063-2. These studies revealed that this gene shares properties of HIV-1 vpr, such as nuclear and virion localization. In addition, SIVagm mutants with inactivating mutations of vpr are unable to replicate in nondividing cells, such as macaque monocyte-derived macrophages, but replicate to almost wild-type levels in a susceptible human T-cell line. The transport of virus preintegration complexes into the nucleus in primary macrophages, as measured by the production of unintegrated circular viral DNA, is less efficient for the mutant viruses than it is for the wild-type virus. SIVagm mutants also replicate inefficiently in primary macaque peripheral blood mononuclear cells, with a propensity for substitutions that remove the inserted inactivating stop cod...Continue Reading

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Citations

Feb 15, 2001·AIDS Research and Human Retroviruses·L JinL Ratner
Jan 28, 2014·PloS One·Susan V WestmorelandRonald C Desrosiers
Jan 11, 2001·Journal of Virology·M P ShermanW C Greene
Oct 24, 2000·Leukemia & Lymphoma·J KulkoskyR J Pomerantz
Jun 7, 2016·Reviews in Medical Virology·Rui SoaresTeresa Gonçalves
Jul 8, 2005·Journal of Leukocyte Biology·Amanda BrownCecilia Cheng-Mayer
Aug 10, 2019·Frontiers in Immunology·Sarah Beth Nodder, Suryaram Gummuluru
Jun 19, 2021·Nature Communications·Li ZhaoGuoxin Liang
Apr 13, 2010·Retrovirology·Diana AyindeFlorence Margottin-Goguet
Jun 3, 2008·Experimental and Molecular Pathology·Joshua L AndersenVicente Planelles

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