Vpr14-88-Apobec3G fusion protein is efficiently incorporated into Vif-positive HIV-1 particles and inhibits viral infection.

PloS One
Zhujun AoXiaojian Yao

Abstract

APOBEC3G (A3G), a deoxycytidine deaminase, is a potent host antiviral factor that can restrict HIV-1 infection. During Vif-negative HIV-1 replication, A3G is incorporated into HIV-1 particles, induces mutations in reverse transcribed viral DNA and inhibits reverse transcription. However, HIV-1 Vif counteracts A3G's activities by inducing its degradation and by blocking its incorporation into HIV-1 particles. Thus, it is interesting to elucidate a mechanism that would allow A3G to escape the effects of Vif in order to rescue its potent antiviral activity and to provide a possible novel therapeutic strategy for treating HIV-1 infection. In this study, we generated an R88-A3G fusion protein by fusing A3G to a virion-targeting polypeptide (R14-88) derived from HIV-1 Vpr protein and compared its antiviral effects relative to those of HA-tagged native A3G (HA-A3G). Our study showed that transient expression of the R88-A3G fusion protein in both Vif(-) and Vif(+) HIV-1 producing cells drastically inhibited viral infection in HeLa-CD4-CCR5-cells, CD4(+) C8166 T cells and human primary PBMCs. Moreover, we established CD4(+) C8166 T cell lines that stably express either R88-A3G or HA-A3G by transduction with VSV-G-pseudotyped lentiviral ...Continue Reading

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H G GöttlingerW A Haseltine
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·M AdamsM Emerman
Apr 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·I W Park, J Sodroski
Dec 1, 1996·Journal of Virology·J GoncalvesD Gabuzda
Aug 15, 1997·The EMBO Journal·T M FletcherB H Hahn
May 28, 1998·The Journal of Biological Chemistry·S PopovM Bukrinsky
May 17, 2003·Science·Denise LecossierAllan J Hance
Jun 18, 2003·Cell·Reuben S HarrisMichael H Malim
Jul 16, 2003·Cell·Roberto MarianiNathaniel R Landau
Oct 7, 2003·Nature Medicine·Mariana MarinDavid Kabat
Nov 15, 2003·Current Biology : CB·Silvestro G ConticelloMichael S Neuberger
Feb 18, 2004·The Journal of Biological Chemistry·Bastien MangeatDidier Trono
Feb 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bärbel SchröfelbauerNathaniel R Landau

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Citations

Jan 26, 2010·Expert Reviews in Molecular Medicine·John S Albin, Reuben S Harris
Feb 7, 2012·The Journal of Biological Chemistry·Zhujun AoXiaojian Yao
Jun 14, 2013·Nucleic Acids Research·Kasandra BélangerMarc-André Langlois
Jul 19, 2011·Journal of Molecular Biology·Elena Britan-RosichMoshe Kotler
May 28, 2013·Journal of Molecular Biology·Jinyu PengXiaojian Yao
Jul 25, 2014·Mobile Genetic Elements·Yujia Cai, Jacob Giehm Mikkelsen
Jan 13, 2018·Microbiology and Molecular Biology Reviews : MMBR·Jean-François GélinasStephen C Hyde

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Methods Mentioned

BETA
ubiquitination
ELISA
FACS
immunoprecipitation
PCR
flow cytometry
fluorescence microscopy
nuclear translocation
FCS
transfection

Software Mentioned

Elispot Reader

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