VvMADS9, a class B MADS-box gene involved in grapevine flowering, shows different expression patterns in mutants with abnormal petal and stamen structures

Functional Plant Biology : FPB
Lekha SreekantanMark R Thomas

Abstract

VvMADS9, a MADS-box gene, from grapevine (Vitis vinifera L.) cultivar Cabernet Sauvignon has been isolated and its expression pattern studied in wild type Cabernet Sauvignon, Mourvèdre, and Bouchalès cultivars and mutants of the latter two genotypes showing abnormal petal / stamen structures. Sequence analysis showed that VvMADS9 was highly similar to PISTILLATA (PI), the class B gene that specifies the identity of petals and stamens in Arabidopsis. The temporal expression pattern of VvMADS9 studied through real-time PCR revealed that its expression was specific to flower development. The low levels of expression in the Mourvèdre mutant and the skewed expression pattern in the Bouchalès mutant as compared to their wild type counterparts suggested that VvMADS9 is involved in normal formation of petals and stamens. Through in situ hybridisation, expression of VvMADS9 was detected in stamens and weak expression on the basal regions of the petals. This suggested a possible role for VvMADS9 in specifying stamen and petal organ identity in grapevine similar to Class B genes in other species. All evidence thus pointed to the conclusion that VvMADS9 is an orthologue of PISTILLATA in grapevine.

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Citations

May 26, 2011·The Plant Journal : for Cell and Molecular Biology·Carmen Dorca-FornellMartin M Kater
Dec 2, 2009·The Plant Journal : for Cell and Molecular Biology·Lucie FernandezJosé M Martinez-Zapater
Nov 19, 2019·PeerJ·João L CoitoMargarida Rocheta
Feb 3, 2007·Plant Molecular Biology·Lucie FernandezAgnès Ageorges
Nov 28, 2012·The Plant Journal : for Cell and Molecular Biology·Lucie FernandezLaurent Torregrosa
Apr 6, 2019·Frontiers in Plant Science·Fabio PalumboGianni Barcaccia
Dec 19, 2013·Journal of Experimental Botany·Xiao HanJae-Yean Kim
Jul 25, 2018·Horticulture Research·Toshi M Foster, Maria José Aranzana
Aug 1, 2018·Frontiers in Plant Science·João L CoitoMargarida Rocheta
Jul 4, 2008·Journal of Experimental Botany·María José CarmonaMark R Thomas
Nov 11, 2008·Plant Physiology·José Díaz-RiquelmeMaría José Carmona

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Datasets Mentioned

BETA
CF406001
CF373459
DQ059750

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

CDART
BLASTX
PROTPARS
Primer3
Conserved Domain Architecture Retrieval Tool ( CDART )

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