PMID: 9005950Jan 15, 1997Paper

vWf inhibitor detection by competitive ELISA

Journal of Immunological Methods
M W StewartP A Gordon

Abstract

An inhibitor to von Willebrand factor (vWf) was detected in the plasma from two patients with histories of mild bleeding and one patient with a severe deficiency in the Factor VIII complex using a competitive enzyme-linked immunosorbent assay (ELISA) procedure. IgG antibodies from the patients' plasmas were shown to bind to vWf immobilised on polystyrene beads by flow cytometry. The inhibitor also potentiated a recently described platelet function assay based on stirring vWf immobilised on polystyrene beads with platelet rich plasma (PRP). Upon addition of mAb IV.3, potentiation of vWf bead-induced platelet activation was lost indicating that the enhancement of platelet activation was Fc receptor-dependent. Since the ELISA described can be used to quantitate vWf and to detect inhibitors to vWf in plasma samples, the method should prove useful in differentiating acquired vWd from congenital vWd.

References

Jan 1, 1978·Journal of Immunological Methods·J W Goding
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M AlgimanM D Kazatchkine
Aug 1, 1986·Thrombosis Research·J E Brown, J O Bosak
Aug 15, 1984·Thrombosis Research·L Thorell, B Blombäck
Dec 1, 1994·Postgraduate Medical Journal·P J Van GenderenF J Huikeshoven
Nov 15, 1994·Thrombosis Research·A BlancoM Lazzari

❮ Previous
Next ❯

Citations

Mar 12, 2002·British Journal of Haematology·A CasonatoA Girolami
Aug 15, 1998·The American Journal of the Medical Sciences·M D Phillips, A Santhouse
Jan 9, 2013·Blood·Paula D JamesPier M Mannucci
Apr 18, 2002·Thrombosis Research·Chitra KrishnamurtiStephen W Rothwell
Nov 13, 2010·Haemophilia : the Official Journal of the World Federation of Hemophilia·M B IrigoyenN Galassi
Mar 31, 1999·Baillière's Clinical Haematology·P J van Genderen, J J Michiels
May 6, 2004·Journal of Biomedical Materials Research. Part a·Yabin ZhuJiacong Shen
Jul 11, 2006·American Journal of Hematology·Hiroshi Mohri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.