WalRK two component system of Bacillus anthracis responds to temperature and antibiotic stress

Biochemical and Biophysical Research Communications
Alisha DhimanRakesh Bhatnagar

Abstract

WalRK Two Component System (TCS) of Bacillus anthracis forms a functional TCS. This report elaborates upon the WalRK genomic architecture, promoter structure, promoter activity and expression under various stress conditions in B. anthracis. 5' RACE located the WalRK functional promoter within 317 bp region upstream of WalR. Reporter gene assays demonstrated maximal promoter activity during early growth phases indicating utility in exponential stages of growth. qRT-PCR showed upregulation of WalRK transcripts during temperature and antibiotic stress. However, WalR overexpression did not affect the tested antibiotic MIC values in B. anthracis. Collectively, these results confirm that WalRK responds to cell envelope stress in B. anthracis.

References

Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Sep 7, 2001·Annual Review of Microbiology·M Mock, A Fouet
Feb 17, 2006·Current Opinion in Microbiology·Dagmar Beier, Roy Gross
Feb 20, 2007·Journal of Bacteriology·Hendrik SzurmantJames A Hoch
Apr 10, 2007·International Journal of Medical Microbiology : IJMM·Andrea JansenGabriele Bierbaum
Jun 22, 2007·Molecular Microbiology·Paola BisicchiaKevin M Devine
Jan 5, 2008·FEMS Microbiology Reviews·Sina JordanThorsten Mascher
Jun 25, 2008·Molecular Microbiology·Sharon Garti-LeviSigal Ben-Yehuda
Nov 8, 2008·Nucleic Acids Research·Fenglou MaoYing Xu
Jul 7, 2009·Annual Review of Microbiology·Rong Gao, Ann M Stock
Jul 4, 2012·Annual Review of Microbiology·Emily J Capra, Michael T Laub

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Citations

Dec 22, 2015·Biochemical and Biophysical Research Communications·Monisha GopalaniRakesh Bhatnagar
Oct 23, 2016·Biochimica Et Biophysica Acta. General Subjects·Somya AggarwalRakesh Bhatnagar
Dec 19, 2016·International Journal of Biological Macromolecules·Alisha DhimanRakesh Bhatnagar
Mar 15, 2018·Journal of Cellular Biochemistry·Vatika GuptaRakesh Bhatnagar

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