Walterinnesia aegyptia venom combined with silica nanoparticles enhances the functioning of normal lymphocytes through PI3K/AKT, NFκB and ERK signaling.

Lipids in Health and Disease
Gamal BadrMaha Daghestani

Abstract

The toxicity of snake venom varies over time in some species. The venom of newborn and small juvenile snakes appears to be more potent than adults of the same species, and a bite from a snake that has not fed recently, such as one that has just emerged from hibernation, is more dangerous than one that has recently fed due to the larger volume of venom injected. Therefore, the potency of a snake's venom is typically determined using the LD50 or IC50 tests. In the present study, we evaluated the anti-tumor potential of snake venom from Walterinnesia aegyptia (WEV) on the human breast carcinoma cell line MDA-MB-231, as well as its effect on the normal mice peripheral blood mononuclear cells (PBMCs). This venom was used alone (WEV) or in combination with silica nanoparticles (WEV+NP). The IC50 values of WEV alone and WEV+NP in the MDA-MB-231 cells were determined to be 50 ng/ml and 20 ng/ml, respectively. Interestingly, at these concentrations, the venom did not affect the viability of normal human PBMCs. To investigate the in vivo effects of this venom further, three groups of mice were used (15 mice in each group): Group I was the control, Group II was subcutaneously injected with WEV, and Group III was injected with WEV+NP. Usin...Continue Reading

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Citations

Dec 15, 2012·Apoptosis : an International Journal on Programmed Cell Death·Gamal BadrDouaa Sayed
Jan 21, 2014·Homeopathy : the Journal of the Faculty of Homeopathy·Paolo BellaviteAnita Conforti
Apr 23, 2014·Global Advances in Health and Medicine : Improving Healthcare Outcomes Worldwide·Iris R BellJohn Ives
Aug 25, 2015·Toxicology and Applied Pharmacology·Sae-Kwang KuJong-Sup Bae
Sep 10, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Quan JinJi-Xing Nan
Feb 17, 2017·European Journal of Haematology·Pilar de la Puente, Abdel Kareem Azab
Oct 4, 2016·International Journal of Immunopathology and Pharmacology·Mohamed H MahmoudNashwa A El Shinnawy
Jul 15, 2017·Journal of Cellular Biochemistry·Min ZhangDaling Zhu
Nov 23, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gamal BadrMohamed H Mahmoud
Feb 8, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Tarek Mohamed Abd El-AzizMichel De Waard
Jan 15, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Nagwa Abo El-MaaliGamal Abd El Wahab
Nov 15, 2016·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Svetlana DinićMelita Vidaković
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jia HeShilin Yang
Nov 21, 2017·Environmental Science and Pollution Research International·Jianhui LiuZhiwei Sun

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Methods Mentioned

BETA
protein assay
FACS
flow cytometry

Software Mentioned

SPSS
NIH ImageJ

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