Warifteine and milonine, alkaloids isolated from Cissampelos sympodialis Eichl: cytotoxicity on rat hepatocyte culture and in V79 cells

Toxicology Letters
Patricia Silva MeloMarcela Haun

Abstract

Two alkaloids were isolated from the leaves of Cissampelos sympodialis; a bisbenzylisoquinoline compound named warifteine and a novel 8,14-dihydromorphinandienone alkaloid named milonine. The cytotoxic effects of these alkaloids were assayed in cultured hepatocytes and V79 fibroblasts. Three independent endpoint assays for cytotoxicity in vitro were used: the nucleic acid content (NAC), tetrazolium reduction (MTT) and neutral red uptake (NRU). Milonine was less toxic than warifteine in both cell cultures. The IC50 values determined in the three different viability assays were around 100 and 400 microM after milonine treatment of V79 cells or hepatocytes. IC50 values ranging from 10 to 35 microM were obtained for warifteine in the viability tests evaluated in V79 cells and hepatocytes. Due to the similar cytotoxic effects detected on V79 cells and hepatocytes, probably warifteine and milonine induced toxic effects independent to the cytochrome P450. This hypothesis was corroborated by the results where Cimetidine (1.0 mM), a traditional cytochrome P450 inhibitor, did not protect the cells from the toxic action of warifteine or milonine. In conclusion, these alkaloids merit further investigations as potential novel pharmacologica...Continue Reading

References

Jan 1, 1994·Antimicrobial Agents and Chemotherapy·S J MarshallP L Schiff
Apr 1, 1996·In Vitro Cellular & Developmental Biology. Animal·J Zurlo, L M Arterburn
Jan 23, 1999·Journal of Natural Products·C K AngerhoferG A Cordell
Apr 12, 2000·Toxicon : Official Journal of the International Society on Toxinology·N RakbaG Lescoat
Sep 21, 2000·Toxicology Letters·P da Silva MeloM Haun
Jun 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A H Hammond, J R Fry
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F PaillardJ A Vericat
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V Bianchi, E Fortunati
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M R CingiF Zucco
Jan 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R JoverM J Gómez-Lechón
Jan 1, 2000·Pharmaceutical Biology·I M SetteM S da-Silva

❮ Previous
Next ❯

Citations

May 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R SethE A Roberts
Oct 29, 2010·Basic & Clinical Pharmacology & Toxicology·Horacinna M M CavalcanteIsac A Medeiros
Aug 31, 2012·Phytotherapy Research : PTR·Giciane C VieiraMarcia R Piuvezam
May 23, 2006·International Immunopharmacology·Cláudio R Bezerra-SantosPatrícia T Bozza
Jul 6, 2014·Journal of Immunology Research·Thaline F A LimaLigia M T Peçanha
Nov 24, 2004·Human & Experimental Toxicology·Patricia Silva MeloMarcela Haun
Apr 13, 2018·Pharmaceutical Biology·Victor Pena RibeiroJairo Kenupp Bastos
Dec 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thaís LisboaMarianna Sobral
Mar 7, 2014·Organic Letters·Bahman Ghavimi, Philip Magnus
Aug 14, 2021·Chemistry & Biodiversity·Jingtao HeChunhua Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.