Warm blood cardioplegic arrest induces mitochondrial-mediated cardiomyocyte apoptosis associated with increased urocortin expression in viable cells

The Journal of Thoracic and Cardiovascular Surgery
Tiziano M ScarabelliJulius M Gardin

Abstract

This study assesses the mechanisms of apoptosis in patients after on-pump coronary artery bypass graft surgery and the potential involvement of the endogenous cardiac peptide urocortin as a cardiomyocyte salvage mechanism. We have previously described the mechanisms of apoptosis after ischemia-reperfusion injury in the rat heart and shown that endogenous urocortin is cardioprotective. Here we extend these findings to the human heart exposed to ischemic-reperfusion injury. Two sequential biopsy specimens were obtained from the right atriums of 24 patients undergoing coronary artery bypass grafting at the start of grafting and 10 minutes after release of the aortic clamp. Apoptosis was identified by means of immunocytochemical colocalization between terminal deoxynucleotidyl transferase-mediated nick end-labeling positivity and active caspase-3. Immunostaining for active caspase-9 and caspase-8 was performed to identify the pathways of apoptosis induction. Urocortin and adenosine triphosphate-dependent potassium channel expression was also assessed by means of immunocytochemistry. Myocyte apoptosis (<0.1% before coronary artery bypass grafting) was increased after coronary artery bypass grafting and reperfusion and was greater in...Continue Reading

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Citations

Jul 5, 2013·Cardiovascular Drugs and Therapy·Francesco OnoratiGiuseppe Faggian
Apr 27, 2010·Molecular and Cellular Endocrinology·Zhaokan YuanTiziano M Scarabelli
Nov 7, 2006·Frontiers in Neuroendocrinology·Eva M Fekete, Eric P Zorrilla
Aug 2, 2005·European Journal of Heart Failure·Carol Chen-ScarabelliTiziano M Scarabelli
Apr 5, 2005·British Journal of Pharmacology·Angel Luis García-VillalónGodofredo Diéguez
Aug 17, 2004·American Heart Journal·Carol Chen-Scarabelli, Tiziano M Scarabelli
Jan 24, 2007·Physiological Reviews·Guido KroemerCatherine Brenner
Jan 1, 2016·Hormones : International Journal of Endocrinology and Metabolism·Efterpi ChouridouEkaterini Chatzaki
Feb 17, 2018·Asian Cardiovascular & Thoracic Annals·Pribadi Wiranda BusroAhmad Aulia Jusuf

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