Warm hepatic ischemia in pigs: effects of L-arginine and oligotide treatment

Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research
P BurraP Rigotti

Abstract

Reperfusion injury represents a key event leading to graft nonfunction. Maintaining adequate nitric oxide levels and stimulating vasodilator synthesis can probably minimize endothelial damage. The aim of this study was to investigate the effect of L-arginine, a substrate of nitric oxide synthesis, and oligotide, a promoter of prostacyclin synthesis, on liver function and morphology after warm ischemia-reperfusion injury. After constructing a side-to-side portacaval shunt, ischemia was induced by clamping the hepatic hilum for 2 h above the shunt, in 19 female pigs divided into a control group (n = 7), an L-arginine treatment group (n = 6), and an oligotide treatment group (n = 6). Liver function tests and measurements of serum and red blood cell malondialdehyde and plasma nitric oxide levels were performed before reperfusion and at 1, 10, 60, and 120 min after reperfusion. Liver biopsies, taken before reperfusion and at 30 min and 7 days after reperfusion, were analyzed for tissue malondialdehyde, histological-ultrastructural features, and apoptosis evaluation. Thirty minutes after reperfusion, liver malondialdehyde, sinusoidal congestion, necrosis, and apoptosis were significantly lower in the L-arginine group than in the cont...Continue Reading

Citations

Dec 1, 2004·Nutrition Research Reviews·Yvonne L J VissersNicolaas E P Deutz
Jun 23, 2009·The Journal of Surgical Research·Rafael Omar GiovanardiAntonio Nocchi Kalil
Sep 23, 2008·BioFactors·Pronobesh ChattopadhyayA K Wahi
Feb 10, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Mahmoud Abu-AmaraBarry Fuller
Dec 2, 2005·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Fernando Lopez-NeblinaLuis H Toledo-Pereyra
Mar 14, 2009·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Rafael Omar GiovanardiAntonio Nocchi Kalil
May 18, 2005·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Heidi SchusterLuc Cynober

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