Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations

Molecular Biology of the Cell
Jeffrey M VerboonSusan M Parkhurst

Abstract

Drosophila immune cells, the hemocytes, undergo four stereotypical developmental migrations to populate the embryo, where they provide immune reconnoitering, as well as a number of non-immune-related functions necessary for proper embryogenesis. Here, we describe a role for Rho1 in one of these developmental migrations in which posteriorly located hemocytes migrate toward the head. This migration requires the interaction of Rho1 with its downstream effector Wash, a Wiskott-Aldrich syndrome family protein. Both Wash knockdown and a Rho1 transgene harboring a mutation that prevents Wash binding exhibit the same developmental migratory defect as Rho1 knockdown. Wash activates the Arp2/3 complex, whose activity is needed for this migration, whereas members of the WASH regulatory complex (SWIP, Strumpellin, and CCDC53) are not. Our results suggest a WASH complex-independent signaling pathway to regulate the cytoskeleton during a subset of hemocyte developmental migrations.

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Citations

Aug 26, 2015·Nucleus·Jeffrey M VerboonSusan M Parkhurst
May 11, 2016·Current Opinion in Cell Biology·Kristen F Swaney, Rong Li
Jun 7, 2020·Journal of Cell Science·Jeffrey M VerboonSusan M Parkhurst
Nov 7, 2019·Nature Cell Biology·Lawrence YollandBrian M Stramer
Apr 19, 2020·Journal of Clinical Immunology·Jeffrey M VerboonVijay G Sankaran
Sep 15, 2020·The Journal of Biological Chemistry·Rebecca R Florke GeePatrick Ryan Potts
Dec 11, 2020·Biochemical Society Transactions·Ewan MacDonaldTobias Zech

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Methods Mentioned

BETA
GTPase
biosensor
GTPases
pull-down
immunoprecipitations
pull downs
transgenic
transgenics
PCR

Software Mentioned

NIS
ImageJ
Volocity
Canvas
Prism
Elements AR
Zeiss LSM

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