PMID: 11934146Apr 6, 2002Paper

Washing of new microscopic glass slides in dichromate solution does not influence sputum AFB smear results

The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
N SelvakumarT Santha

Abstract

Microscopy centres in Tiruvallur District, Tamil Nadu, India, implementing DOTS. To know whether washing new glass slides in dichromate solution is essential for effective sputum acid-fast bacilli microscopy. Two direct smears were prepared from each of 1750 sputum samples. One was made on dichromate solution-cleaned new glass slides and the other was made on unwashed new glass slides. The smears were blinded and examined. Of the 1750 specimens, 13.5% and 13.08% were positive for AFB using washed and unwashed slides, respectively (P = 0.12). The concordance between these two (including one grade above and one below) was 98.7%. Washing of new glass slides in dichromate solution is not essential for AFB microscopy.

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