Washing stored red blood cells in an albumin solution improves their morphologic and hemorheologic properties

Transfusion
W H ReinhartSergey S Shevkoplyas

Abstract

Prolonged storage of red blood cells (RBCs) leads to storage lesions, which may impair clinical outcomes after transfusion. A hallmark of storage lesions is progressive echinocytic shape transformation, which can be partially reversed by washing in albumin solutions. Here we have investigated the impact of this shape recovery on biorheologic variables. RBCs stored hypothermically for 6 to 7 weeks were washed in a 1% human serum albumin (HSA) solution. RBC deformability was measured with osmotic gradient ektacytometry. The viscosity of RBC suspensions was measured with a Couette-type viscometer. The flow behavior of RBCs suspended at 40% hematocrit was tested with an artificial microvascular network (AMVN). Washing in 1% albumin reduced higher degrees of echinocytes and increased the frequency of discocytes, thereby shifting the morphologic index toward discocytosis. Washing also reduced RBC swelling. This shape recovery was not seen after washing in saline, buffer, or plasma. RBC shape normalization did not improve cell deformability measured by ektacytometry, but it tended to decrease suspension viscosities at low shear rates and improved the perfusion of an AMVN. Washing of stored RBCs in a 1% HSA solution specifically reduce...Continue Reading

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Citations

May 5, 2016·Transfusion·Marc CloutierSonia Néron
Oct 22, 2016·Clinical Hemorheology and Microcirculation·Walter H Reinhart
Feb 19, 2016·Clinical Hemorheology and Microcirculation·M TarasevR Davenport
Jul 7, 2017·Transfusion·Nathaniel Z PietySergey S Shevkoplyas
Sep 21, 2016·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Walter H ReinhartSergey S Shevkoplyas
Apr 18, 2018·Clinical Hemorheology and Microcirculation·Jean-Frédéric BrunEric Raynaud de Mauverger
Aug 13, 2017·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Walter H ReinhartSergey S Shevkoplyas
Dec 30, 2017·American Journal of Hematology·Eszter VörösSergey S Shevkoplyas
Apr 25, 2018·Transfusion·Sarah GehrkeAngelo D'Alessandro
Aug 17, 2019·Cytotherapy·Rui LiAllison Hubel
Dec 24, 2021·Journal of Veterinary Internal Medicine·Ashley C CollJohn M Thomason

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