Water and solute permeabilities of medullary thick ascending limb apical and basolateral membranes

The American Journal of Physiology
R L RiversMark L Zeidel

Abstract

The medullary thick ascending limb (MTAL) reabsorbs solute without water and concentrates NH4+ in the interstitium without a favorable pH gradient, activities which require low water and NH3 permeabilities. The contributions of different apical and basolateral membrane structures to these low permeabilities are unclear. We isolated highly purified apical and basolateral MTAL plasma membranes and measured, by stopped-flow fluorometry, their permeabilities to water, urea, glycerol, protons, and NH3. Osmotic water permeability at 20 degrees C averaged 9.4 +/- 0.8 x 10(-4) cm/s for apical and 11.9 +/- 0.5 x 10(-4) cm/s for basolateral membranes. NH3 permeabilities at 20 degrees C averaged 0.0023 +/- 0.00035 and 0.0035 +/- 0.00080 cm/s for apical and basolateral membranes, respectively. These values are consistent with those obtained in isolated perfused tubules and can account for known aspects of MTAL function in vivo. Because the apical and basolateral membrane unit permeabilities are similar, the ability of the apical membrane to function as the site of barrier function arises from its very small surface area when compared with the highly redundant basolateral membrane.

References

May 11, 1992·The American Journal of Physiology·E B GrossmanM L Zeidel
Jun 8, 1989·Nature·D KikeriS C Hebert
Jan 1, 1987·The Journal of Membrane Biology·K Strange, K R Spring
Feb 1, 1985·The Journal of Clinical Investigation·L L HammJ Buerkert
Mar 1, 1973·The American Journal of Physiology·M B Burg, N Green
Oct 1, 1984·The American Journal of Anatomy·B C KoneC C Tisher
Nov 1, 1994·The American Journal of Physiology·A ChangM L Zeidel
Aug 1, 1994·The American Journal of Physiology·M B LandeM L Zeidel
Jan 11, 1994·The American Journal of Physiology·A I PiquerasM L Zeidel
May 17, 1996·The Journal of Biological Chemistry·H O NegreteM L Zeidel
Sep 1, 1996·The Journal of Membrane Biology·M M Timbs, K R Spring

❮ Previous
Next ❯

Citations

Aug 15, 2001·American Journal of Physiology. Cell Physiology·G ChandyT E Machen
Feb 8, 2008·American Journal of Physiology. Renal Physiology·Bryce MaciverMark L Zeidel
Feb 25, 2005·American Journal of Physiology. Cell Physiology·Warren G HillMark L Zeidel
Jun 4, 2005·The Journal of Physiology·Volker Endeward, Gerolf Gros
Nov 20, 2009·American Journal of Physiology. Renal Physiology·Alan M Weinstein
Nov 20, 2009·American Journal of Physiology. Renal Physiology·Alan M Weinstein, Thomas A Krahn
Sep 13, 2001·American Journal of Physiology. Renal Physiology·R ChambreyM Paillard
Oct 26, 2012·American Journal of Physiology. Renal Physiology·Aniel Nieves-GonzálezLeon C Moore
Nov 18, 2011·American Journal of Physiology. Renal Physiology·Pascal Houillier, Soline Bourgeois
Feb 6, 2017·Physiological Reviews·I David Weiner, Jill W Verlander
Feb 25, 2005·American Journal of Physiology. Renal Physiology·Joshua D ZeidelMark L Zeidel
Nov 13, 2002·Kidney International·Dominique EladariFrançoise Leviel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.