Water channels are important for osmotic adjustments of yeast cells at low temperature

Microbiology
Graça SoveralTeresa F Moura

Abstract

The importance of aquaporin expression in water permeability in Saccharomyces cerevisiae was assessed by measuring the osmotic water permeability coefficient (P(f)) and the activation energies (E(a)) from both hypo- and hypertonic experiments performed with whole protoplasts from four strains differing in aquaporin level of expression: parental, double-deleted and overexpressing AQY1 or AQY2. Double-deleted (lower P(f)) and AQY1-overexpressing strains (higher P(f)) presented linear Arrhenius plots with E(a) consistent with fluxes mainly through the lipids [16.3 kcal mol(-1) (68.2 kJ mol(-1))] and with a strong contribution of channels [9.6 kcal mol(-1) (40.2 kJ mol(-1))], respectively. The Arrhenius plots for the parental (swelling experiments) and overexpressing AQY2 strains (swelling and shrinking experiments) were not linear, presenting a break point with a change in slope around 23 degrees C. The E(a) values for these strains, calculated for temperatures ranging from 7 to 23 degrees C, were lower [9.5 kcal mol(-1) (39.7 kJ mol(-1))] than the values obtained from 23 to 38 degrees C [17 kcal mol(-1) (71.1 kJ mol(-1))]. This behaviour indicates that only in the lower temperature range did the water fluxes occur predominantly v...Continue Reading

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Citations

Nov 8, 2008·Plant & Cell Physiology·Guo-Wei LiWei-Ai Su
Feb 6, 2007·Applied and Environmental Microbiology·Graça SoveralTeresa F Moura
Feb 8, 2014·PloS One·Iliana PereiraMaria José Leandro
Sep 13, 2014·Applied Microbiology and Biotechnology·Uwe Nehls, Sandra Dietz
Feb 13, 2016·Frontiers in Chemistry·Ana MadeiraGraça Soveral
Feb 7, 2012·Fungal Genetics and Biology : FG & B·Joanne Wong Sak HoiJean-Paul Latgé
Sep 12, 2008·Cell Calcium·Jiangjun CuiKyle W Cunningham
Dec 15, 2010·Biology of the Cell·Graça SoveralMaria C Loureiro-Dias
Oct 1, 2013·Biochimica Et Biophysica Acta·Doryaneh AhmadpourStefan Hohmann
Jun 20, 2014·Biochemical and Biophysical Research Communications·Luís LeitãoGraça Soveral
Aug 19, 2008·Biochimica Et Biophysica Acta·Graça SoveralTeresa F Moura
Apr 22, 2017·FEMS Microbiology Letters·Farzana SabirCatarina Prista
Nov 6, 2018·Current Protein & Peptide Science·João P Bezerra-NetoAna M Benko-Iseppon
Jun 18, 2020·International Journal of Molecular Sciences·Mohamed FaizeJean-Stéphane Venisse
Jan 22, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Carlos SaldañaTeresa García-Gasca
May 20, 2017·Microbiology Spectrum·Mark D FrickerLynne Boddy

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