Water diffusion in bicelles and the mixed bicelle model

Langmuir : the ACS Journal of Surfaces and Colloids
Ronald Soong, Peter Macdonald

Abstract

To test a prediction of the mixed bicelle model, stimulated echo (STE) pulsed field gradient (PFG) (1)H nuclear magnetic resonance (NMR) measurements of water diffusion between and across bicellar lamellae were performed in positively and negatively magnetically aligned bicelles, composed of mixtures of DHPC (1,2-dihexanoyl-sn-glycero-3-phosphocholine) and DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine), as a function of temperature and of the proportion of added short-chain lipid DHPC. (31)P NMR spectra obtained for each situation confirmed that the DHPC undergoes fast exchange between curved and planar regions as per the mixed bicelle model and permitted an estimate of the proportion of the two DHPC populations. Water diffusion across the bicellar lamellae was shown to scale directly with q*, the fraction of edge versus planar phospholipid, rather than simply the ratio q, the global fraction of long-chain to short-chain phospholipid. Geometric modeling of the dependence of water diffusion on q* suggested an upper limit of 400 A for the size of DHPC-rich toroidal perforations within the bicelle lamellae. These findings constitute an independent confirmation of the mixed bicelle model in which DHPC is not confined to edge re...Continue Reading

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Citations

Mar 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Mu-Ping NiehPeter Macdonald
Jan 24, 2012·Molecular Pharmaceutics·Gelen RodríguezOlga López
Jan 10, 2012·Molecular Pharmaceutics·Zhenwei LuCharles R Sanders
Sep 27, 2012·Physical Chemistry Chemical Physics : PCCP·Gelen RodríguezOlga López
Dec 28, 2012·European Biophysics Journal : EBJ·Gelen RodríguezMercedes Cócera
Mar 8, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Ulrich H N DürrAyyalusamy Ramamoorthy
Jun 17, 2010·Biophysical Journal·Yong JiangJames T Kindt
Aug 5, 2009·Biophysical Journal·Ronald SoongPeter M Macdonald
Nov 27, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Hannah Hazel MoralesPeter M Macdonald
Feb 6, 2018·Biophysical Journal·Emmanuel O Awosanya, Alexander A Nevzorov
Sep 9, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Gelen RodríguezOlga López
Apr 13, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Gelen RodríguezOlga López
Feb 10, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ronald SoongPeter M Macdonald
Sep 26, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ming LiMu-Ping Nieh
Dec 27, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Alanna FlynnMichael R Morrow

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