Water dynamics in glycosphingolipid aggregates studied by LAURDAN fluorescence

Biophysical Journal
L BagatolliG D Fidelio

Abstract

We have characterized the fluorescence properties of 6-dodecanoyl-2-dimethylamine-naphthalene (LAURDAN) in pure interfaces formed by sphingomyelin and 10 chemically related glycosphingolipids (GSLs).1 The GSLs contain neutral and anionic carbohydrate residues in their oligosaccharide chain. These systems were studied at temperatures below, at, or above the main phase transition temperature of the pure lipid aggregates. The extent of solvent dipolar relaxation around the excited fluorescence probe in the GSLs series increases with the magnitude of the glycosphingolipid polar headgroup below the transition temperature. This conclusion is based on LAURDAN's excitation generalized polarization (GPex) and fluorescence lifetime values found in the different interfaces. A linear dependence between the LAURDAN GPex and the intermolecular spacing among the lipid molecules was found for both neutral and anionic lipids in the GSLs series. This relationship was also followed by phospholipids. We conclude that LAURDAN in these lipid aggregates resides in sites containing different amounts of water. The dimension of these sites increases with the size of the GSLs polar headgroup. The GP function reports on the concentration and dynamics of w...Continue Reading

References

Aug 23, 1979·Biochimica Et Biophysica Acta·W I Calhoun, G G Shipley
Jun 1, 1990·Biophysical Journal·T ParasassiE Gratton
Jun 24, 1987·Biochimica Et Biophysica Acta·W Curatolo
Dec 1, 1982·Chemistry and Physics of Lipids·D BachI R Miller
Sep 30, 1980·Biochimica Et Biophysica Acta·Y Barenholz, T E Thompson
Jan 1, 1994·Progress in Biophysics and Molecular Biology·B Maggio
Mar 1, 1995·Journal of Fluorescence·E Gratton, T Parasassi
Mar 1, 1995·Journal of Fluorescence·T Parasassi, E Gratton
Sep 1, 1992·Journal of Fluorescence·T Parasassi, E Gratton

❮ Previous
Next ❯

Citations

Apr 17, 2009·Cellular and Molecular Life Sciences : CMLS·Gurunadh R Chichili, William Rodgers
Jun 23, 2006·Journal of Fluorescence·Cíntia C De Vequi-SuplicyM Teresa Lamy
Feb 12, 2013·Journal of Fluorescence·Cintia C Vequi-SuplicyCássia A Marquezin
Feb 11, 2000·Chemistry and Physics of Lipids·M M PincelliA M Gennaro
Jul 10, 2002·Trends in Immunology·Daniel M Davis
Oct 6, 2009·The Journal of Biological Chemistry·Isabella ParoliniStefano Fais
Oct 5, 2010·Traffic·Mahassine SafouanePatricia Bassereau
Jun 15, 2005·Biochimica Et Biophysica Acta·Fernanda RicchelliSergio Bova
Dec 14, 2004·Chemistry and Physics of Lipids·Juha M HolopainenPaavo K J Kinnunen
Sep 2, 2006·Biochimica Et Biophysica Acta·Juha-Matti AlakoskelaPaavo K J Kinnunen
Aug 12, 2008·Biophysical Journal·Berenice VenegasParkson Lee-Gau Chong
Apr 16, 2009·Biochimica Et Biophysica Acta·Karin A RiskeM Teresa Lamy
May 17, 2012·Biochimica Et Biophysica Acta·A Srinivas ReddyMirianas Chachisvilis
Jan 26, 2011·Biochimica Et Biophysica Acta·Anders BjörkbomJ Peter Slotte
Jan 5, 2011·Biochimica Et Biophysica Acta·Christian SergeliusShigeo Katsumura
Mar 17, 2010·Biochimica Et Biophysica Acta·Jonathan BrewerLuis A Bagatolli
Dec 10, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Patrick DrückerHans-Joachim Galla
Jan 28, 2017·Biochimica Et Biophysica Acta. Biomembranes·Jonathan BrewerLuis A Bagatolli
Nov 27, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Vicente Castro-CastilloGerman Gunther
Mar 30, 2017·Methods and Applications in Fluorescence·Olga RyzhovaPaavo Kinnunen
Jul 5, 2001·The Journal of Biological Chemistry·S A WaschukJ A McLaurin
Apr 14, 2006·Molecular Membrane Biology·Katharina GausThomas Harder
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Danuta Gutowska-OwsiakJorge Bernardino de la Serna
Aug 24, 2005·Biochemistry·Hasna AhyayauchFélix M Goñi
Aug 30, 2008·The Journal of Physical Chemistry. B·Ranjan DasYves Mély
Aug 1, 2021·Acta Biomaterialia·Kei NishidaMasaru Tanaka
Jan 23, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Dibyendu Kumar SasmalKankan Bhattacharyya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.