PMID: 12765763May 27, 2003Paper

Water-gated mechanism of proton translocation by cytochrome c oxidase

Biochimica Et Biophysica Acta
Mårten WikströmGerhard Hummer

Abstract

Cytochrome c oxidase is essential for aerobic life as a membrane-bound energy transducer. O(2) reduction at the haem a(3)-Cu(B) centre consumes electrons transferred via haem a from cytochrome c outside the membrane. Protons are taken up from the inside, both to form water and to be pumped across the membrane (M.K.F. Wikström, Nature 266 (1977) 271; M. Wikström, K. Krab, M. Saraste, Cytochrome Oxidase, A Synthesis, Academic Press, London, 1981 ). The resulting electrochemical proton gradient drives ATP synthesis (P. Mitchell, Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation, Glynn Research, Bodmin, UK, 1966 ). Here we present a molecular mechanism for proton pumping coupled to oxygen reduction that is based on the unique properties of water in hydrophobic cavities. An array of water molecules conducts protons from a conserved glutamic acid, either to the Delta-propionate of haem a(3) (pumping), or to haem a(3)-Cu(B) (water formation). Switching between these pathways is controlled by the redox-state-dependent electric field between haem a and haem a(3)-Cu(B), which determines the water-dipole orientation, and therefore the proton transfer direction. Proton transfer via the propionate provides a gate to O(2)...Continue Reading

References

Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell
Dec 1, 1994·Journal of Bioenergetics and Biomembranes·J E MorganM Wikström
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·M L VerkhovskayaM Wikström
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·A Puustinen, M Wikström
Sep 27, 2000·Biochimica Et Biophysica Acta·M WikströmM I Verkhovsky
Mar 5, 2003·Biochimica Et Biophysica Acta·Xuehe ZhengAlexei A Stuchebrukhov
Apr 12, 2003·Physical Review Letters·Christoph DellagoGerhard Hummer

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Citations

Sep 4, 2013·Amino Acids·Dragan M Popović
Nov 1, 2008·Journal of Bioenergetics and Biomembranes·Peter Brzezinski, Robert B Gennis
Jan 2, 2013·Journal of Biological Physics·Pascale Mentré
Apr 9, 2005·Trends in Biochemical Sciences·Artur OsyczkaP Leslie Dutton
Nov 6, 2010·Chemical Reviews·Sharon Hammes-Schiffer, Alexei A Stuchebrukhov
Jan 13, 2006·The Journal of Physical Chemistry. a·P H KönigQ Cui
Apr 14, 2007·The Journal of Physical Chemistry. B·Jessica M J SwansonGregory A Voth
Jun 28, 2011·Physical Chemistry Chemical Physics : PCCP·Ville R I Kaila, Gerhard Hummer
Dec 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Dmitry BlochMårten Wikström
Jul 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dmitry ZaslavskyRobert B Gennis
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric PiletMarten H Vos
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Subramanian VaitheeswaranGerhard Hummer
Apr 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Robert B Best, Gerhard Hummer
Apr 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jiancong Xu, Gregory A Voth
Jul 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mårten WikströmAnne Puustinen
Nov 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lina SalomonssonPeter Brzezinski
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gisela BrändénPeter Brzezinski
Apr 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mats H M Olsson, Arieh Warshel
Oct 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Laura S BusenlehnerRichard N Armstrong
Feb 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ilya BelevichMichael I Verkhovsky
Jun 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Francesco FornasieroOlgica Bakajin
May 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Andrei V PisliakovArieh Warshel
Apr 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ville R I KailaMårten Wikström
Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ida Namslauer, Peter Brzezinski
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Young C KimGerhard Hummer
May 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Amandine Maréchal, Peter R Rich
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Andreas NamslauerPeter Brzezinski
Oct 24, 2007·Antioxidants & Redox Signaling·Ilya Belevich, Michael I Verkhovsky
Sep 11, 2009·Antioxidants & Redox Signaling·Giorgio Lenaz, Maria Luisa Genova
Jul 19, 2013·Journal of the Royal Society, Interface·Peter R Rich, Amandine Maréchal
May 20, 2005·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Per E M Siegbahn, Margareta R A Blomberg
Jul 29, 2006·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christopher C MoserP Leslie Dutton
Jun 8, 2006·Annual Review of Biochemistry·Jonathan P HoslerDenise A Mills
Oct 28, 2008·Biological Chemistry·Sayan Gupta, Shyamalava Mazumdar
Jun 25, 2009·The Journal of Chemical Physics·Anatoly Yu SmirnovFranco Nori
May 7, 2011·Annual Review of Biophysics·Shinya YoshikawaKyoko Shinzawa-Itoh
Feb 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Young C KimGerhard Hummer
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