PMID: 9177749Jun 1, 1997Paper

Water (H2O and D2O) molar absorptivity in the 1000-4000 cm-1 range and quantitative infrared spectroscopy of aqueous solutions

Analytical Biochemistry
Venyaminov SYu, F G Prendergast

Abstract

Water (H2O and D2O) molar absorptivity was measured by Fourier transform infrared transmission spectroscopy in the 1000-4000 cm-1 range at 25 degrees C. A series of assembled cells with path lengths from 1.2 to 120.5 microns was used for these measurements. The optimal path length (the path length of aqueous solution at which the IR spectrum of solute, corrected for water absorbance, has the highest signal-to-noise ratio) was calculated for all water absorbance bands. The results presented here show that the optimal path length does not depend on solute properties and is inversely proportional to the solvent (water) molar absorptivity. The maximal signal-to-noise ratio for measurements of IR spectra of aqueous solution in the 1650 cm-1 spectral region, of primary interest in biological applications, can be obtained at an optimal cell path lengths of 3-4 microns (H2O) and 40-60 microns (D2O). As an example, the signal-to-noise ratio was calculated as a function of the cell path length for the amide I (H2O) and amide I' (D2O) bands of an aqueous lysozyme solution. The molar absorptivities of water bands are several orders of magnitude weaker than those of the strongest bands of biological macromolecules in the same spectral regio...Continue Reading

Citations

Sep 30, 2008·Biochemistry·Andrei K Dioumaev, Janos K Lanyi
Apr 8, 2010·The Journal of Physical Chemistry. B·Binghua Chai, Gerald H Pollack
Oct 16, 2009·The Journal of Physical Chemistry. B·Binghua ChaiGerald H Pollack
Nov 15, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Min Zhang, Yuqing Wu
May 5, 2007·The Review of Scientific Instruments·Martin SchiewekAlfred Blume
Sep 21, 2013·The Journal of Chemical Physics·Aurélie LasoroskiRodolphe Pollet
May 19, 2007·The Journal of Chemical Physics·Jean-Joseph MaxCamille Chapados
Nov 18, 2009·The Journal of Chemical Physics·Jean-Joseph Max, Camille Chapados
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S D ZakharovW A Cramer
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Wei-Tao Liu, Y Ron Shen
Jan 6, 2016·The Journal of Physical Chemistry. B·Chiara H GiammancoMichael D Fayer
Jan 23, 2016·Structural Dynamics·Maria EkimovaErik T J Nibbering
Sep 10, 2016·The Journal of Chemical Physics·Luigi De MarcoAndrei Tokmakoff
Sep 13, 2016·Journal of Chemical Theory and Computation·Tatsuya Joutsuka, Akihiro Morita
Nov 1, 2016·Applied Spectroscopy·Ariel Bohman, Mark A Arnold
Mar 10, 2017·Journal of the American Chemical Society·Paul Stevenson, Andrei Tokmakoff
Apr 21, 2017·Journal of Inorganic Biochemistry·Florian BrulfertÉric Simoni
Apr 13, 2017·Physical Chemistry Chemical Physics : PCCP·Jianbo MaDaniel G Kuroda
Feb 7, 2006·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Martina Wolpert, Petra Hellwig
Oct 3, 2006·Journal of Colloid and Interface Science·Thierry RichardLouis d'Hendecourt
Mar 24, 2017·Physical Chemistry Chemical Physics : PCCP·Marianna AmbricoMarco d'Ischia
Jan 27, 2016·Physical Chemistry Chemical Physics : PCCP·Andy WongEvan G Robertson
Feb 21, 2008·Applied Spectroscopy·Y Raichlin, A Katzir
Oct 11, 2016·Physical Chemistry Chemical Physics : PCCP·Elena RagnoniAndrea Lapini
Aug 14, 2015·Soft Matter·Shao-Hua WuNoah Malmstadt
Jul 18, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Seyed Meysam Hashemnejad, Santanu Kundu
Oct 22, 2015·Chemical Society Reviews·K L Andrew Chan, Sergei G Kazarian
Mar 4, 2017·Scientific Reports·Charlotte K RousePall Thordarson
Jan 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haoqian LiDeguo Du
Oct 1, 2008·Physical Chemistry Chemical Physics : PCCP·Michael A KambouresR Benny Gerber
Oct 19, 2005·Journal of Biomedical Optics·Heinz FabianDieter Naumann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.