Water penetration and binding to ferric myoglobin

Biochemistry
W CaoJ T Sage

Abstract

Flash photolysis investigations of horse heart metmyoglobin bound with NO (Mb(3+)NO) reveal the kinetics of water entry and binding to the heme iron. Photodissociation of NO leaves the sample in the dehydrated Mb(3+) (5-coordinate) state. After NO photolysis and escape, a water molecule enters the heme pocket and binds to the heme iron, forming the 6-coordinate aquometMb state (Mb(3+)H2O). At longer times, NO displaces the H2O ligand to reestablish equilibrium. At 293 K, we determine a value k(w) approximately 5.7 x 10(6) s(-1) for the rate of H2O binding and estimate the H2O dissociation constant as 60 mM. The Arrhenius barrier height H(w) = 42 +/- 3 kJ/mol determined for H2O binding is identical to the barrier for CO escape after photolysis of Mb(2+)CO, within experimental uncertainty, consistent with a common mechanism for entry and exit of small molecules from the heme pocket. We propose that both processes are gated by displacement of His-64 from the heme pocket. We also observe that the bimolecular NO rebinding rate is enhanced by 3 orders of magnitude both for the H64L mutant, which does not bind water, and for the H64G mutant, where the bound water is no longer stabilized by hydrogen bonding with His-64. These results e...Continue Reading

References

May 1, 1992·Scientific American·S H Snyder, D S Bredt
Oct 28, 1966·Journal of Molecular Biology·M F Perutz, F S Mathews
Jan 1, 1995·Annual Review of Physiology·J Garthwaite, C L Boulton
Nov 5, 1993·Journal of Molecular Biology·M L QuillinG N Phillips
Mar 8, 1996·Journal of Molecular Biology·F Yang, G N Phillips
Apr 25, 1997·Journal of Molecular Biology·V P DenisovB Halle
Dec 22, 1999·Journal of Cardiovascular Pharmacology·L J IgnarroC Napoli

❮ Previous
Next ❯

Citations

Aug 17, 2004·Biosensors & Bioelectronics·Hui-Hong LiuDai-Wen Pang
Apr 21, 2005·Journal of the American Chemical Society·Xiong YePaul M Champion
Feb 2, 2006·Journal of the American Chemical Society·Xiong YePaul M Champion
Mar 22, 2008·Journal of the American Chemical Society·Flaviu GruiaPaul M Champion
Aug 7, 2009·Journal of the American Chemical Society·Robert A GoldbeckRaymond M Esquerra
Feb 4, 2010·Journal of the American Chemical Society·Abdelkrim BenabbasPaul M Champion
Mar 21, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jaeheung ParkManho Lim
Jan 10, 2012·The Journal of Biological Chemistry·Byung-Kuk YooMichel Negrerie
Mar 25, 2008·The Journal of Biological Chemistry·Raymond M EsquerraRobert A Goldbeck
Jan 21, 2014·The Journal of Chemical Physics·Cristina ConsaniMajed Chergui
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert A GoldbeckRaymond M Esquerra
Aug 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Marius SchmidtVukica Srajer
Mar 12, 2013·The Journal of Physical Chemistry. B·Weiqiao ZengPaul M Champion
Nov 4, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Susanna Herold, Alain Puppo
Jun 20, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gianantonio BattistuzziMarco Sola
Dec 15, 2015·The Journal of Physical Chemistry Letters·Jean-Christophe LambryMarten H Vos
Dec 3, 2013·The Protein Journal·Balakrishnan Vijayakumar, Devadasan Velmurugan
Jul 3, 2007·Biophysical Journal·Ana DamjanovićBertrand García-Moreno E
Dec 11, 2007·Biophysical Journal·Flaviu GruiaPaul M Champion
Sep 29, 2004·Journal of Structural Biology·Friedrich SchottePhilip A Anfinrud
Mar 14, 2008·Journal of Inorganic Biochemistry·M Laura FernándezSara E Bari
Jun 30, 2016·Biochemistry·Raymond M EsquerraRobert A Goldbeck
Sep 19, 2019·Antioxidants & Redox Signaling·Fernando Martín BoubetaSara Elizabeth Bari
Aug 13, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Leonardo Angeloni, Alessandro Feis
Sep 19, 2019·Physical Chemistry Chemical Physics : PCCP·Mohammad AarabiNiloufar Shafizadeh
Aug 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Camila BacellarMajed Chergui
Apr 9, 2019·Metallomics : Integrated Biometal Science·Michel Négrerie
Apr 11, 2002·Chemical Reviews·Peter C Ford, Ivan M Lorkovic
Aug 15, 2008·Journal of the American Chemical Society·Morten Køcks LykkegaardHenning Zettergren
Apr 24, 2021·Journal of Inorganic Biochemistry·Jaime E Martinez GrundmanJuliette T J Lecomte
Jul 30, 2010·Inorganic Chemistry·Peter C Ford
Nov 8, 2013·The Journal of Physical Chemistry. B·Shuji Kaieda, Bertil Halle
May 16, 2014·The Journal of Physical Chemistry. B·Venugopal KarunakaranPaul M Champion
Feb 26, 2013·The Journal of Physical Chemistry. B·Jaeheung ParkManho Lim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.