Water-soluble extract of Salvia miltiorrhiza ameliorates carbon tetrachloride-mediated hepatic apoptosis in rats

The Journal of Pharmacy and Pharmacology
Tzung-Yan LeeHan-Chieh Lin

Abstract

Apoptosis is one of the events that are involved in liver fibrogenesis. Thus, factors that affect apoptosis may be used to modulate liver fibrosis. We have recently reported that Salvia miltiorrhiza plays a protective role in carbon tetrachloride (CCl4)-induced hepatic fibrosis. In this study, we aimed to evaluate whether S. miltiorrhiza modulated CCl4-induced hepatic apoptosis in rats. Male Wistar rats were given orally either vehicle or water-extract of S. miltiorrhiza (50 mg kg(-1) twice daily) for nine weeks beginning from the start of CCl4 administration. A group of normal rats was included for comparison. Hepatocyte DNA fragmentation and cytosolic caspase-3 and caspase-8 activity were determined in the experimental animals. Hepatic cytosolic Bax, Bcl-2, cytochrome c, and calpain-mu expressions were measured by Western blot analysis. Hepatic mitochondrial glutathione levels were assessed by colorimetric assay. Compared with normal rats, rats receiving CCl4 alone showed profound DNA fragmentation associated with an increased cytosolic fraction of cytochrome c and calpain-mu protein expressions and a decreased mitochondrial glutathione level. In contrast, a decreased laddering of DNA fragmentation was noted in rats receiving...Continue Reading

References

Nov 3, 1995·The Journal of Biological Chemistry·T MiyashitaJ C Reed
May 1, 1994·Journal of Cellular Physiology·M K SquìerJ J Cohen
Nov 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F OberhammerR Schulte-Hermann
Jan 1, 1994·The Journal of Cell Biology·J C Reed
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K SaitoR A Nixon
Aug 1, 1997·Journal of Hepatology·P R Galle
Feb 7, 1998·The Biochemical Journal·H SorimachiK Suzuki
Aug 28, 1998·Science·D R Green, J C Reed
Oct 2, 1998·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·P Calès
Dec 2, 1998·Journal of Hepatology·S WasserC E Tan
Jul 17, 2001·Biochemical and Biophysical Research Communications·E MezeyJ J Potter
Apr 2, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rolando Hernández-MuñozMyrna A R Dent
Jan 10, 2004·The Journal of Pharmacy and Pharmacology·Tzung-Yan LeeHan-Chieh Lin
Jun 14, 2005·Gut·M E Guicciardi, G J Gores

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Citations

Aug 22, 2009·Chinese Medicine·Yibin FengYao Tong
Jul 13, 2011·International Journal of Molecular Sciences·Yun-Chen TienWen-Huang Peng
Jul 11, 2013·World Journal of Gastroenterology : WJG·Xiao-Ling GuoQin Cao
Jan 27, 2016·Journal of Pharmacological Sciences·Xi ChuYong-sheng Dong
Jan 19, 2016·Frontiers in Pharmacology·Ralf Weiskirchen
Jun 16, 2014·Journal of the Chinese Medical Association : JCMA·Zi-Yu ChangHen-Hong Chang
Aug 22, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Eun-Jeon ParkDong Hwan Sohn
Apr 14, 2007·The Journal of Pharmacy and Pharmacology·Tzung-Yan LeeHan-Chieh Lin
Dec 24, 2014·Environmental Toxicology·Hamdy A A AlyMohamed F Abd-Ellah
Mar 27, 2013·Environmental Toxicology and Pharmacology·Min-Cheol KangYou-Jin Jeon
May 9, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Supaluk PrachayasittikulVirapong Prachayasittikul
May 9, 2015·World Journal of Gastroenterology : WJG·Bo LiangZheng-Quan Feng
Jan 11, 2019·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Qianrui ZhangHongping Song

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