Water-soluble nicotinic acetylcholine receptor formed by alpha7 subunit extracellular domains.

The Journal of Biological Chemistry
Gregg B WellsJ Lindstrom

Abstract

Water-soluble models of ligand-gated ion channels would be advantageous for structural studies. We investigated the suitability of three versions of the N-terminal extracellular domain (ECD) of the alpha7 subunit of the nicotinic acetylcholine receptor (AChR) family for this purpose by examining their ligand-binding and assembly properties. Two versions included the first transmembrane domain and were solubilized with detergent after expression in Xenopus oocytes. The third was truncated before the first transmembrane domain and was soluble without detergent. For all three, their equilibrium binding affinities for alpha-bungarotoxin, nicotine, and acetylcholine, combined with their velocity sedimentation profiles, were consistent with the formation of native-like AChRs. These characteristics imply that the alpha7 ECD can form a water-soluble AChR that is a model of the ECD of the full-length alpha7 AChR.

References

May 1, 1992·Brain Research. Molecular Brain Research·K Sumikawa
Mar 1, 1991·The Journal of Cell Biology·M S SaediJ Lindstrom
Jul 15, 1971·European Journal of Biochemistry·E KarlssonD Eaker
Jan 1, 1984·Methods in Enzymology·M WilchekJ Kohn
Mar 31, 1995·Science·G A BentleyR A Mariuzza
May 19, 1995·The Journal of Biological Chemistry·P J CorringerD Bertrand
Sep 1, 1994·Brain Research. Molecular Brain Research·K Sumikawa, T Nishizaki
Jan 1, 1993·Annual Review of Biochemistry·P T Englund
Feb 20, 1993·Journal of Molecular Biology·N Unwin
Apr 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M E MedofM L Tykocinski

❮ Previous
Next ❯

Citations

Oct 16, 1999·Toxicon : Official Journal of the International Society on Toxinology·Y N UtkinV I Tsetlin
Jun 6, 2002·European Journal of Biochemistry·Victor I TsetlinChristoph Methfessel
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·P J CorringerJ P Changeux
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Gregg B Wells
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·M FischerJ P Changeux
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nicolas Le NovèreJean-Pierre Changeux
Sep 2, 2006·The EMBO Journal·Yury BogdanovStephen J Moss
Apr 6, 2016·Scientific Reports·Akash PandhareMichaela Jansen
Sep 22, 2005·The Journal of Biological Chemistry·Alexandra M PersonGregg B Wells
Jul 27, 2007·The FEBS Journal·Dimitra KalamidaSocrates J Tzartos
Aug 15, 2019·The Journal of General Physiology·Akash PandhareMichaela Jansen
Nov 18, 2009·Journal of Molecular Biology·Hugues NuryMarc Delarue
May 19, 2001·Molecular and Cellular Neurosciences·J F Leite, M Cascio

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.