Water transport by aquaporins in the extant plant Physcomitrella patens

Plant Physiology
David LiénardJean-Paul Lassalles

Abstract

Although aquaporins (AQPs) have been shown to increase membrane water permeability in many cell types, the physiological role of this increase was not always obvious. In this report, we provide evidence that in the leafy stage of development (gametophore) of the moss Physcomitrella patens, AQPs help to replenish more rapidly the cell water that is lost by transpiration, at least if some water is in the direct vicinity of the moss plant. Three AQP genes were cloned in P. patens: PIP2;1, PIP2;2, and PIP2;3. The water permeability of the membrane was measured in protoplasts from leaves and protonema. A significant decrease was measured in protoplasts from leaves and protonema of PIP2;1 or PIP2;2 knockouts but not the PIP2;3 knockout. No phenotype was observed when knockout plants were grown in closed petri dishes with ample water supply. Gametophores isolated from the wild type and the pip2;3 mutant were not sensitive to moderate water stress, but pip2;1 or pip2;2 gametophores expressed a water stress phenotype. The knockout mutant leaves were more bent and twisted, apparently suffering from an important loss of cellular water. We propose a model to explain how the AQPs PIP2;1 and PIP2;2 delay leaf dessication in a drying atmosphe...Continue Reading

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Citations

Apr 24, 2008·BMC Plant Biology·Jonas A H Danielson, Urban Johanson
Sep 3, 2008·Trends in Plant Science·Daniel LangRalf Reski
Oct 11, 2012·The Plant Journal : for Cell and Molecular Biology·Yuki YasumuraNicholas P Harberd
Jan 29, 2014·Plant Science : an International Journal of Experimental Plant Biology·Maria del Carmen Martinez-Ballesta, Micaela Carvajal
Oct 15, 2009·Molecular Plant·Audra J Charron, Ralph S Quatrano
Sep 7, 2016·Plant Science : an International Journal of Experimental Plant Biology·Boaz Negin, Menachem Moshelion
Nov 8, 2016·Frontiers in Plant Science·Hemasundar AlavilliSanjib Kumar Panda
Oct 16, 2016·Plant, Cell & Environment·Michael GroszmannJohn R Evans
Feb 4, 2016·Journal of Developmental Biology·Zunaira AfzalM Shahid Mukhtar
Aug 9, 2016·Plant, Cell & Environment·Christophe MaurelOlivier Rodrigues

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