WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge.

Molecular Biology of the Cell
Qing TangBruce L Goode

Abstract

SCAR/WAVE proteins and Arp2/3 complex assemble branched actin networks at the leading edge. Two isoforms of SCAR/WAVE, WAVE1 and WAVE2, reside at the leading edge, yet it has remained unclear whether they perform similar or distinct roles. Further, there have been conflicting reports about the Arp2/3-independent biochemical activities of WAVE1 on actin filament elongation. To investigate this in vivo, we knocked out WAVE1 and WAVE2 genes, individually and together, in B16-F1 melanoma cells. We demonstrate that WAVE1 and WAVE2 are redundant for lamellipodia formation and motility. However, there is a significant decrease in the rate of leading edge actin extension in WAVE2 KO cells, and an increase in WAVE1 KO cells. The faster rates of actin extension in WAVE1 KO cells are offset by faster retrograde flow, and therefore do not translate into faster lamellipodium protrusion. Thus, WAVE1 restricts the rate of actin extension at the leading edge, and appears to couple actin networks to the membrane to drive protrusion. Overall, these results suggest that WAVE1 and WAVE2 have redundant roles in promoting Arp2/3-dependent actin nucleation and lamellipodia formation, but distinct roles in controlling actin network extension and harne...Continue Reading

References

Aug 1, 1991·The Journal of Cell Biology·M H Symons, T J Mitchison
Jun 25, 1999·Biochemical and Biophysical Research Communications·S SuetsuguT Takenawa
Nov 27, 2001·Science·R C RobinsonT D Pollard
Feb 9, 2002·Science·Naoki Watanabe, Timothy J Mitchison
Feb 23, 2002·Trends in Cell Biology·J Victor SmallKlemens Rottner
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Matthew D Welch, R Dyche Mullins
Dec 17, 2002·Journal of Cell Science·Motohiro NozumiShigeaki Miyamoto
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott H SoderlingJohn D Scott
Jul 15, 2003·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Khalid Sossey-AlaouiJohn K Cowell
Jul 23, 2003·Nature Structural Biology·Sanjay C PanchalMichael K Rosen
Jul 25, 2003·Nature·Daisuke YamazakiTadaomi Takenawa
Feb 7, 2004·The EMBO Journal·Anika SteffenTheresia E B Stradal
Mar 9, 2005·BMC Cell Biology·Craig F StovoldLaura M Machesky
Apr 20, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Daisuke YamazakiTadaomi Takenawa
Nov 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·David ChereauRoberto Dominguez
Apr 7, 2006·Molecular Biology of the Cell·Anika SteffenTheresia E B Stradal
May 26, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hyun-Ju KimTimothy K Vartanian
Aug 11, 2006·The Journal of Biological Chemistry·Jeremy R StuartZhi-Min Yuan
Jan 12, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Scott H SoderlingJohn D Scott
May 5, 2007·Annual Review of Biophysics and Biomolecular Structure·Thomas D Pollard
Jun 5, 2007·Nature Methods·Benjamin J ZeskindPaul Matsudaira
Nov 23, 2007·Journal of Cell Science·Christopher M DansonGiles O Cory
Mar 1, 2008·The EMBO Journal·Frank P L LaiKlemens Rottner
May 9, 2008·Structure·Malgorzata BoczkowskaRoberto Dominguez
Aug 30, 2008·Journal of Microscopy·J BlockJ Faix
Dec 17, 2008·The Journal of Cell Biology·Margaret L GardelClare M Waterman
Jun 16, 2009·Cell·Jose M SilvaGregory J Hannon
Mar 20, 2010·Nature Reviews. Molecular Cell Biology·Kenneth G Campellone, Matthew D Welch
Jun 11, 2010·Annual Review of Biochemistry·Shae B Padrick, Michael K Rosen
Dec 2, 2010·The Journal of Biological Chemistry·Hiroshi YamashitaNoriyuki Kioka
Mar 23, 2011·Molecular Cell·Michelle C MendozaJohn Blenis
Jun 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shih-Chieh TiThomas D Pollard
Jun 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shae B PadrickMichael K Rosen
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Stefan LinderMirko Himmel
Jan 1, 2013·Current Biology : CB·Haoran TangLaura M Machesky
Jan 5, 2013·Science·Le CongFeng Zhang
Jul 24, 2013·Annual Review of Cell and Developmental Biology·Sandrine Etienne-Manneville
Jul 26, 2013·Molecular Biology of the Cell·Stefan A KoestlerKlemens Rottner

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Methods Mentioned

BETA
electron microscopy
fluorescence recovery after photobleaching
transfection

Software Mentioned

NIS element analysis
ImageJ
VisiView
NIS
Excel
NIS elements
Chemotaxis and Migration Tool
GraphPad
MetaVi
GraphPad Prism

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