WAVE1 is associated with invasiveness and growth of prostate cancer cells

The Journal of Urology
H S FernandoWen G Jiang

Abstract

WAVE1 belongs to the Wiskott-Aldrich syndrome family of proteins, which have an integral part in cell motility, a crucial step in cancer metastasis. We investigated the expression pattern and the effects of manipulating endogenous WAVE1 expression in prostate cancer cells. WAVE1 protein expression in normal and cancer specimens, and in prostate cell lines was assessed using immunohistochemistry and reverse transcriptase-polymerase chain reaction, respectively. Hammerhead ribozyme transgenes were synthesized and cloned into the mammalian expression vector pEF6/V5-His TOPO TA, and transfected by electroporation into PC-3(DeltaW1R1/2) and DU-145(DeltaW1R1/2) cell lines. In vitro invasion, adhesion and growth assays were used to assess the impact of WAVE1 knockdown. Immunohistochemistry of prostate tissue specimens showed that the cytoplasm of cancer cells had stronger staining than normal epithelium. Reverse transcriptase-polymerase chain reaction for WAVE1 showed strong expression in the PC-3 (European Collection of Cell Cultures, Salisbury, United Kingdom) and DU-145 (ATCC(R)) cell lines. WAVE1 knockdown was associated with a significant decrease in invasion but not in adhesion. The mean +/- SEM number of invading PC-3(DeltaW1R1...Continue Reading

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Citations

Feb 18, 2011·Nature Reviews. Cancer·Alexander NürnbergRobert Grosse
Jul 19, 2012·Journal of Cancer Research and Clinical Oncology·Yi ZhangChun-Yi Hao
Feb 2, 2011·Leukemia & Lymphoma·Ming-Hua YangLi-Zhi Cao
Dec 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sima AzizmohammadiSusan Azizmohammadi
Aug 21, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·K M SakthivelC Guruvayoorappan
Oct 16, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Wen G JiangKeith G Harding
Dec 8, 2017·Physiological Reviews·Nicolas Molinie, Alexis Gautreau
Apr 25, 2015·Oncotarget·Adam G SowalskyLeszek Kotula

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