Wax-tear and meibum protein, wax-β-carotene interactions in vitro using infrared spectroscopy.

Experimental Eye Research
Samad FaheemDouglas Borchman

Abstract

Protein-meibum and terpenoids-meibum lipid interactions could be important in the etiology of meibomian gland dysfunction (MGD) and dry eye symptoms. In the current model studies, attenuated total reflectance (ATR) infrared (IR) spectroscopy was used to determine if the terpenoid β-carotene and the major proteins in tears and meibum affect the hydrocarbon chain conformation and carbonyl environment of wax, an abundant component of meibum. The main finding of these studies is that mucin binding to wax disordered slightly the conformation of the hydrocarbon chains of wax and caused the wax carbonyls to become hydrogen bonded or experience a more hydrophilic environment. Lysozyme and lactoglobulin, two proteins shown to bind to monolayers of meibum, did not have such an effect. Keratin and β-carotene did not affect the fluidity (viscosity) or environment of the carbonyl moieties of wax. Based on these results, tetraterpenoids are not likely to influence the structure of meibum in the meibomian glands. In addition, these findings suggest that it is unlikely that keratin blocks meibomian glands by causing the meibum to become more viscous. Among the tear fluid proteins studied, mucin is the most likely to influence the conformation ...Continue Reading

References

Sep 1, 1978·Experimental Eye Research·J M Tiffany
Mar 1, 1991·Chemistry and Physics of Lipids·H H Mantsch, R N McElhaney
Nov 1, 1989·Archives of Biochemistry and Biophysics·P Di MascioH Sies
May 10, 1973·Experimental Eye Research·F J Holly
Dec 4, 1984·Biochimica Et Biophysica Acta·H L Casal, H H Mantsch
Sep 1, 1981·Survey of Ophthalmology·N J Van Haeringen
Sep 1, 1982·American Journal of Ophthalmology·V J GutgesellC I Hood
May 1, 1995·Current Eye Research·B J GlasgowW L Hubbell
Jul 1, 1994·Cornea·D R KorbJ V Greiner
Oct 1, 1993·Optometry and Vision Science : Official Publication of the American Academy of Optometry·P Cho, M Yap
Mar 1, 1993·Current Eye Research·C K ChewA J Bron
Jun 17, 1998·Experimental Eye Research·D TangR J Cenedella
Jul 31, 1998·Archives of Ophthalmology·W E Shine, J P McCulley
Aug 14, 1998·Experimental Eye Research·D Tang, D Borchman
Oct 20, 1998·Progress in Retinal and Eye Research·K Tsubota
Sep 4, 1999·Ophthalmic Research·D TangM C Yappert
Jan 13, 2000·Cornea·W E Shine, J P McCulley
Apr 16, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Norhani MohidinMaurice Yap
Apr 26, 2003·Investigative Ophthalmology & Visual Science·Daxin TangVittorio Rasi
May 6, 2003·Biochimica Et Biophysica Acta·A G Lee
Oct 27, 2004·Cornea·Ward E Shine, James P McCulley
Nov 16, 2004·Phytochemistry·Etienne AchitouvClaude Largeau
Jan 12, 2005·Experimental Eye Research·Douglas BorchmanMuhammad Afzal
Jun 22, 2005·Experimental Eye Research·Vahid GramiDouglas Borchman
Jul 12, 2005·Colloids and Surfaces. B, Biointerfaces·F MianoV Enea
Dec 16, 2005·Current Eye Research·C J Radke
Feb 21, 2006·The British Journal of Ophthalmology·P S TsaiD A Sullivan
Mar 15, 2006·Colloids and Surfaces. B, Biointerfaces·Poonam MudgilThomas J Millar
Nov 30, 2006·The Ocular Surface·Alan Tomlinson, Santosh Khanal
Jun 19, 2007·Survey of Ophthalmology·Gary N Foulks
Dec 21, 2007·Journal of Lipid Research·Lora C RoboskyMichael D Reily
Feb 22, 2008·Experimental Eye Research·Poonam Mudgil, Thomas J Millar
Feb 26, 2008·Biochimica Et Biophysica Acta·Derek Marsh
Aug 5, 2008·Optometry and Vision Science : Official Publication of the American Academy of Optometry·P Ewen King-SmithRichard J Braun
Sep 2, 2008·Investigative Ophthalmology & Visual Science·Thomas J MillarChendur K Palaniappan

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Citations

Jun 19, 2013·Experimental Eye Research·Igor A Butovich
May 20, 2015·Experimental Eye Research·Thomas J Millar, Burkhardt S Schuett
May 22, 2019·Investigative Ophthalmology & Visual Science·Douglas BorchmanGary N Foulks
Aug 2, 2019·Cornea·Douglas BorchmanAparna Ramasubramanian
Nov 9, 2018·International Journal of Molecular Sciences·Poonam MudgilAparna Ramasubramanian
Jun 20, 2020·Journal of Lipid Research·Douglas Borchman
Feb 9, 2021·Journal of Lipid Research·Douglas Borchman
Dec 10, 2020·BMJ Open Ophthalmology·Aparna RamasubramanianDouglas Borchman
Apr 14, 2021·Experimental Eye Research·Simin MasoudiMark D Willcox
Jun 25, 2021·Advanced Drug Delivery Reviews·Matthias MarczynskiOliver Lieleg

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