WBC alloimmunization: effects on the laboratory and clinical endpoints of therapeutic granulocyte transfusions

Transfusion
Thomas H PriceS F Assmann

Abstract

Although the subject of many previous studies, the importance of white blood cell (WBC) alloimmunization in granulocyte transfusion therapy has not been settled. In this study, we report the results of the effects of WBC antibodies in the RING (Resolving Infection in Neutropenia with Granulocytes) study, a randomized controlled trial comparing the efficacy of daily granulocyte transfusion therapy plus antimicrobials versus antimicrobials alone; the primary outcome results have been published previously. One hundred fourteen subjects were enrolled in the study. Serum samples for WBC antibody determination were obtained from each subject at baseline and at 2 and 6 weeks. One hundred subjects had at least one antibody test result. Samples were tested for human leukocyte antigen (HLA) Class I and Class II antibodies as well as for granulocyte-specific antibodies using granulocyte agglutination and immunofluorescence techniques. All testing was performed at a central laboratory. Baseline WBC alloimmunization was modest, depending somewhat on the assay. Seroconversion during the study was slightly higher in the granulocyte transfusion arm, but the differences were not statistically significant. There was no demonstrable effect of the...Continue Reading

References

Aug 1, 1977·British Journal of Haematology·F W VerheugtC P Engelfriet
Sep 1, 1977·Transfusion·F R AppelbaumR G Graw
Apr 1, 1990·American Journal of Hematology·J P DutcherC A Schiffer
Nov 1, 1996·Transfusion·D F StroncekS F Leitman
Oct 28, 2011·Manual Therapy·Christopher Kevin Wong
Jan 28, 2014·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Parastoo B DahiJuliet N Barker

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Citations

Jun 5, 2019·Transfusion·Juergen Bux, Ulrich J Sachs
Oct 10, 2019·Transfusion·Maureen J MillerJohn D Roback
Dec 2, 2018·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Torsten J Schulze, Peter Bugert

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