Abstract
Streptomyces griseus S4-7 was originally isolated from the strawberry rhizosphere as a microbial agent responsible for Fusarium wilt suppressive soils. S. griseus S4-7 shows specific and pronounced antifungal activity against Fusarium oxysporum f. sp. fragariae. In the Streptomyces genus, the whi transcription factors are regulators of sporulation, cell differentiation, septation, and secondary metabolites production. wblE2 function as a regulator has emerged as a new group in whi transcription factors. In this study, we reveal the involvement of the wblE2 transcription factor in the plant-protection by S. griseus S4-7. We generated ΔwblE, ΔwblE2, ΔwhiH, and ΔwhmD gene knock-out mutants, which showed less antifungal activity both in vitro and in planta. Among the mutants, wblE2 mutant failed to protect the strawberry against the Fusarium wilt pathogen. Transcriptome analyses revealed major differences in the regulation of phenylalanine metabolism, polyketide and siderophore biosynthesis between the S4-7 and the wblE2 mutant. The results contribute to our understanding of the role of streptomycetes wblE2 genes in a natural disease suppressing system.
References
Jul 1, 1992·Gene·M BiermanB E Schoner
Jun 1, 1983·Canadian Journal of Microbiology·J A GuijarroC Hardisson
Dec 3, 1984·European Journal of Biochemistry·K MikulíkA Jiránová
Aug 1, 1997·Science·H BeinertE Münck
Mar 9, 2000·Microbiology·J A SoliveriK F Chater
Mar 27, 2003·Molecular Microbiology·Keith F Chater, Sueharu Horinouchi
May 7, 2003·Current Opinion in Microbiology·Patricia J Kiley, Helmut Beinert
Jun 5, 2004·Journal of Microbiological Methods·W Van DesselJ Anné
Nov 24, 2004·Current Opinion in Microbiology·Marie A Elliot, Nicholas J Talbot
Dec 24, 2004·The Journal of Biological Chemistry·Piotr JakimowiczMark J Buttner
Apr 2, 2005·Current Opinion in Microbiology·Mervyn J Bibb
Feb 17, 2007·Molecular Microbiology·Md Suhail AlamPushpa Agrawal
Feb 12, 2008·BMC Genomics·Ramy K AzizOlga Zagnitko
Dec 17, 2008·Nature Reviews. Microbiology·Klas Flärdh, Mark J Buttner
Dec 4, 2009·Biochemistry·Jason C CrackNick E Le Brun
Mar 4, 2010·Genome Biology·Mark D Robinson, Alicia Oshlack
Jun 16, 2010·Molecular Microbiology·Jan RybnikerPia Hartmann
Oct 12, 2010·The Biochemical Journal·Laura J SmithJeffrey Green
Nov 10, 2011·The Journal of Biological Chemistry·Ján BurianCharles J Thompson
Nov 19, 2011·FEMS Microbiology Reviews·Joseph R McCormick, Klas Flärdh
Jan 11, 2012·Antimicrobial Agents and Chemotherapy·Gabriela NovotnaHee-Jeon Hong
Jan 14, 2012·Antioxidants & Redox Signaling·Jason C CrackNick E Le Brun
Mar 19, 2013·FEMS Microbiology Letters·Paula YagüeAngel Manteca
Nov 22, 2013·Biochemical Society Transactions·Mervyn J Bibb
Dec 7, 2013·BMC Microbiology·Paola SalernoKlas Flärdh
Jun 10, 2015·The ISME Journal·Jae-Yul ChaYoun-Sig Kwak
Mar 31, 2017·Organic & Biomolecular Chemistry·Qingfei ZhengWen Liu
Citations
Jun 19, 2019·FEMS Microbiology Letters·Sung Won HongYoun-Sig Kwak
Oct 10, 2018·MicrobiologyOpen·Liang WangZhonggui Mao
Jan 5, 2021·Natural Product Reports·Namil LeeByung-Kwan Cho
Apr 17, 2020·The Plant Pathology Journal·Jisu KimYoun-Sig Kwak