wblE2 transcription factor in Streptomyces griseus S4-7 plays an important role in plant protection

MicrobiologyOpen
Hyun Ji ChoYoun-Sig Kwak

Abstract

Streptomyces griseus S4-7 was originally isolated from the strawberry rhizosphere as a microbial agent responsible for Fusarium wilt suppressive soils. S. griseus S4-7 shows specific and pronounced antifungal activity against Fusarium oxysporum f. sp. fragariae. In the Streptomyces genus, the whi transcription factors are regulators of sporulation, cell differentiation, septation, and secondary metabolites production. wblE2 function as a regulator has emerged as a new group in whi transcription factors. In this study, we reveal the involvement of the wblE2 transcription factor in the plant-protection by S. griseus S4-7. We generated ΔwblE, ΔwblE2, ΔwhiH, and ΔwhmD gene knock-out mutants, which showed less antifungal activity both in vitro and in planta. Among the mutants, wblE2 mutant failed to protect the strawberry against the Fusarium wilt pathogen. Transcriptome analyses revealed major differences in the regulation of phenylalanine metabolism, polyketide and siderophore biosynthesis between the S4-7 and the wblE2 mutant. The results contribute to our understanding of the role of streptomycetes wblE2 genes in a natural disease suppressing system.

References

Jun 1, 1983·Canadian Journal of Microbiology·J A GuijarroC Hardisson
Dec 3, 1984·European Journal of Biochemistry·K MikulíkA Jiránová
Mar 27, 2003·Molecular Microbiology·Keith F Chater, Sueharu Horinouchi
May 7, 2003·Current Opinion in Microbiology·Patricia J Kiley, Helmut Beinert
Jun 5, 2004·Journal of Microbiological Methods·W Van DesselJ Anné
Nov 24, 2004·Current Opinion in Microbiology·Marie A Elliot, Nicholas J Talbot
Dec 24, 2004·The Journal of Biological Chemistry·Piotr JakimowiczMark J Buttner
Apr 2, 2005·Current Opinion in Microbiology·Mervyn J Bibb
Feb 12, 2008·BMC Genomics·Ramy K AzizOlga Zagnitko
Dec 17, 2008·Nature Reviews. Microbiology·Klas Flärdh, Mark J Buttner
Mar 4, 2010·Genome Biology·Mark D Robinson, Alicia Oshlack
Nov 10, 2011·The Journal of Biological Chemistry·Ján BurianCharles J Thompson
Nov 19, 2011·FEMS Microbiology Reviews·Joseph R McCormick, Klas Flärdh
Jan 11, 2012·Antimicrobial Agents and Chemotherapy·Gabriela NovotnaHee-Jeon Hong
Jan 14, 2012·Antioxidants & Redox Signaling·Jason C CrackNick E Le Brun
Mar 19, 2013·FEMS Microbiology Letters·Paula YagüeAngel Manteca
Nov 22, 2013·Biochemical Society Transactions·Mervyn J Bibb
Jun 10, 2015·The ISME Journal·Jae-Yul ChaYoun-Sig Kwak
Mar 31, 2017·Organic & Biomolecular Chemistry·Qingfei ZhengWen Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

edgeR
R
ggplot2
CLRNASeq
gplots
SigmaPlot
RAST
TRizol

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.