WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells

Animal Biotechnology
Yoonjung KhoYunjaie Choi

Abstract

In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNFalpha treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNFalpha induced WDNM1 expression showed the NFkappaB-dependent mechanism since it's expression was abrogated in IkappaBalphaM (super-repressor of NFkappaB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNFalpha-NFkappaB signal pathway, is associated with HC11 cell apoptosis.

References

Apr 15, 1991·Biochemical and Biophysical Research Communications·T N Dear, R F Kefford
May 1, 1989·The American Journal of Anatomy·N I WalkerJ F Kerr
Jul 5, 1996·Biochemical and Biophysical Research Communications·M LeeM Baik
Jul 1, 1996·The Journal of Cell Biology·S GrimmK Schulze-Osthoff
Nov 1, 1996·Science·D J Van AntwerpI M Verma
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·S HunotE C Hirsch
Nov 14, 1997·Acta Oto-laryngologica·S Y KimT E Carey
Nov 21, 1997·Biochemical and Biophysical Research Communications·M LeeM Baik
Apr 14, 1999·Cell Death and Differentiation·M Grilli, M Memo
Apr 29, 2000·Nature·K M RyanK H Vousden
Aug 31, 2000·Annual Review of Biochemistry·A StrasserV M Dixit
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y ZhengA A Beg
Sep 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T M BaetuJ Hiscott
Oct 27, 2001·The Journal of Biological Chemistry·X BianA W Opipari
Feb 26, 2003·Investigative Ophthalmology & Visual Science·Ping YangGlenn J Jaffe

❮ Previous
Next ❯

Citations

Nov 6, 2014·Journal of Cellular Biochemistry·William A FreijeSherin U Devaskar

❮ Previous
Next ❯

Datasets Mentioned

BETA
AF159701

Methods Mentioned

BETA
PCR

Software Mentioned

Cell Quest
Softmax

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis