WE-C-217BCD-05: A Novel Interpolation Method for the 3D Reconstruction of Cell Structures

Medical Physics
Y SaY Feng

Abstract

To develop a new interpolation method for accurate 3D reconstruction of cell morphology from laser scanning confocal microscope (LSCM) image data. Current techniques are based on the assumption that pixel intensity or contour shapes of images change linearly in the interpolation direction. Gray-value and position of the pixel in interpolated image slice are obtained through weighted average calculation with gray-values and distances of corresponding pixels in two adjacent original image slices, only information from adjacent image slices is considered, often fail to meet the need of 3D reconstruction for cells because of the complex cell morphology. The new method interpolates cellular organelle contours in polar coordinate system. Coordinate system origin is chosen to be the mass center weighted by pixel intensity instead of conventional geometric center, contour points of the organelle is sampled by their angles first and fitted with uniform cubic B-spline to perform interpolation. For complex organelle structures such as branched nuclei, a special method combining morphological information and corner detection technique based on curvature scale space has been developed to solve the contour division and related problems. New ...Continue Reading

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