Weak bases increase surface IgM expression in 70Z/3 B lymphoid cell line without increasing kappa gene expression

Cellular Immunology
J OstrowskiK Bomsztyk

Abstract

A murine B lymphoid cell line, 70Z/3, has proven to be a useful model to study the developmental transition from membrane immunoglobulin (mIgM)-negative to mIgM-positive B cells. 70Z/3 cells have normal intracellular levels of mu heavy chain but kappa mRNA is transcribed at a very low rate. Because both kappa and mu chains are required for IgM to localize on the external membrane the cells remain essentially mIgM negative. A number of biologic agents can stimulate kappa mRNA transcription leading to increased surface IgM expression. Pharmacologic agents which cause cytoplasmic alkalinization have also been shown to increase the level of surface IgM expression when assessed by FITC-labeled anti-IgM antibodies. This increase has been used to argue that cytoplasmic alkalinization is sufficient to stimulate 70Z/3 differentiation but previous studies did not measure kappa mRNA levels or rate of synthesis. In this study we set out to determine whether the increased FITC staining that follows cytoplasmic alkalinization results from increased surface IgM expression and whether that increase results from activation of kappa mRNA transcription. When cells were treated with methylamine, NH4Cl, or monensin there was transient cytoplasmic a...Continue Reading

References

Jan 1, 1988·Annual Review of Cell Biology·M L Atchison
Jan 1, 1988·The American Journal of Physiology·K BomsztykT H Stanton
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H E Ives, T O Daniel
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M A ValentineE A Clark
Jan 1, 1983·The Journal of Cell Biology·R M SteinmanZ A Cohn
Jan 1, 1984·Immunology Letters·T Stanton, M Maynard
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P M Rosoff, L C Cantley
Apr 1, 1980·Cell·E L Reinherz, S F Schlossman

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