Weak D Testing is not Required for D- Patients With C-E- Phenotype

Annals of Laboratory Medicine
Sooin ChoiD Cho

Abstract

Although testing to detect weak D antigens using the antihuman globulin reagent is not required for D- patients in many countries, it is routinely performed in Korea. However, weak D testing can be omitted in D- patients with a C-E- phenotype as this indicates complete deletion of the RHD gene, except in rare cases. We designed a new algorithm for weak D testing, which consisted of RhCE phenotyping followed by weak D testing in C+ or E+ samples, and compared it with the current algorithm with respect to time and cost-effectiveness. In this retrospective study, 74,889 test results from January to July 2017 in a tertiary hospital in Korea were analyzed. Agreement between the current and proposed algorithms was evaluated, and total number of tests, time required for testing, and test costs were compared. With both algorithms, RHD genotyping was conducted for samples that were C+ or E+ and negative for weak D testing. The algorithms showed perfect agreement (agreement=100%; κ=1.00). By applying the proposed algorithm, 29.56% (115/389 tests/yr) of tests could be omitted, time required for testing could be reduced by 36% (8,672/24,084 min/yr), and the test cost could be reduced by 16.53% (536.11/3,241.08 USD/yr). Our algorithm omitti...Continue Reading

References

Feb 5, 2010·The New England Journal of Medicine·Chao-Peng Shao
Feb 26, 2013·British Journal of Haematology·Geoff Daniels
Dec 3, 2014·Transfusion·S Gerald SandlerUNKNOWN College of American Pathologists Transfusion Medicine Resource Committee Work Group
Dec 30, 2014·Turkish Journal of Haematology : Official Journal of Turkish Society of Haematology·Diana RemeikieneElona Juozaityte
Jun 3, 2015·Transfusion Medicine·M WangX J Wan
May 17, 2017·British Journal of Haematology·S Gerald SandlerWilly A Flegel

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Citations

Feb 29, 2020·Acta Clinica Belgica·Sreelekshmi SPoornima Baliga B

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Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

it
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