Weeds as Potential Inoculum Reservoir for Colletotrichum nymphaeae Causing Strawberry Anthracnose in Iran and Rep-PCR Fingerprinting as Useful Marker to Differentiate C. acutatum Complex on Strawberry

Frontiers in Microbiology
Kaivan KarimiIlaria Pertot

Abstract

Strawberry anthracnose caused by Colletotrichum spp. is considered one of the most serious and destructive disease of strawberry worldwide. Weeds, as possible hosts of the pathogen, could have a role as potential inoculum reservoir. To prove this hypothesis, symptomless weeds were collected in strawberry fields showing anthracnose symptoms in Iran. Ten isolates with Colletotrichum-like colonies were recovered from symptomless Amaranthus viridis L., Convolvulus arvensis L., Fumaria officinalis L., Lactuca serriola L., and Sonchus oleraceus L. plants. The isolates were identified as C. nymphaeae, based on a combination of morphological and sequence data of TUB and GADPH genes. This identification was further validated using Rep-PCR fingerprinting analysis, which produces species-specific DNA fingerprints and unveils inter and intra variation of the species examined in this study. Moreover, rep-PCR marker was used to reveal accurate taxonomic position of Colletorichum spp. causing strawberry anthracnose belonging to the C. acutatum complex, including C. acutatum sensu stricto, C. fiorinae, C. godetiae, C. nymphaeae, C. salicis, and C. simmondsii. The C. nymphaeae isolates originating from symptomless weeds confirmed their pathogen...Continue Reading

References

Apr 1, 1995·Applied and Environmental Microbiology·N L Glass, G C Donaldson
Jun 1, 1996·Molecular Biotechnology·R Staden
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Oct 9, 2004·Journal of Clinical Microbiology·Patricio GodoyArnaldo Lopes Colombo
Feb 13, 2007·Research in Microbiology·Artur AlvesAntónio Correia
Apr 5, 2012·Molecular Plant Pathology·Ralph DeanGary D Foster
Nov 9, 2012·Studies in Mycology·U DammP W Crous
Nov 9, 2012·Studies in Mycology·U DammP W Crous
Nov 9, 2012·Studies in Mycology·B S WeirU Damm
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 5, 2015·Persoonia·F LiuU Damm

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Citations

Mar 25, 2020·Microbial Biotechnology·Youming ShenHaifei Li
Oct 5, 2020·Journal of Applied Microbiology·M BonacciG Barros

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Methods Mentioned

BETA
PCR

Software Mentioned

GeneRuler
Staden
Muscle
FigTree
NTSYS
UPGMA
SAS
BLAST
PC
imageJ

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