Weevil pgrp-lb prevents endosymbiont TCT dissemination and chronic host systemic immune activation

Proceedings of the National Academy of Sciences of the United States of America
Justin MaireAbdelaziz Heddi

Abstract

Long-term intracellular symbiosis (or endosymbiosis) is widely distributed across invertebrates and is recognized as a major driving force in evolution. However, the maintenance of immune homeostasis in organisms chronically infected with mutualistic bacteria is a challenging task, and little is known about the molecular processes that limit endosymbiont immunogenicity and host inflammation. Here, we investigated peptidoglycan recognition protein (PGRP)-encoding genes in the cereal weevil Sitophilus zeamais's association with Sodalis pierantonius endosymbiont. We discovered that weevil pgrp-lb generates three transcripts via alternative splicing and differential regulation. A secreted isoform is expressed in insect tissues under pathogenic conditions through activation of the PGRP-LC receptor of the immune deficiency pathway. In addition, cytosolic and transmembrane isoforms are permanently produced within endosymbiont-bearing organ, the bacteriome, in a PGRP-LC-independent manner. Bacteriome isoforms specifically cleave the tracheal cytotoxin (TCT), a peptidoglycan monomer released by endosymbionts. pgrp-lb silencing by RNAi results in TCT escape from the bacteriome to other insect tissues, where it chronically activates the h...Continue Reading

References

Aug 1, 1995·Molecular Microbiology·J T Park
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A HeddiP Nardon
Mar 1, 1967·Journal of Theoretical Biology·L Sagan
Apr 16, 2002·Science·Ruslan Medzhitov, Charles A Janeway
Dec 21, 2002·Molecular Microbiology·Ahmed El ZoeibyRoger C Levesque
Apr 15, 2003·Nature Immunology·François LeulierBruno Lemaitre
Jan 24, 2004·Molecular Biology and Evolution·Cédric LefèvreAbdelaziz Heddi
Nov 13, 2004·Science·Tanya A KoropatnickMargaret J McFall-Ngai
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carolyn R StenbakDominique Mengin-Lecreulx
Apr 19, 2006·Immunity·Anna Zaidman-RémyBruno Lemaitre
Aug 29, 2006·Journal of Molecular Biology·Clyde A Smith
Oct 6, 2006·Applied and Environmental Microbiology·Caroline AnselmeAbdelaziz Heddi
Jan 5, 2007·Annual Review of Immunology·Bruno Lemaitre, Jules Hoffmann
Mar 17, 2007·Nature Reviews. Microbiology·Julien Royet, Roman Dziarski
May 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nancy A Moran
Apr 9, 2008·Journal of Bacteriology·Daniel L Garcia, Joseph P Dillard
Oct 18, 2008·BMC Biology·Caroline AnselmeAbdelaziz Heddi
May 19, 2009·BMC Biotechnology·Agnès VallierAbdelaziz Heddi
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Petr HruzLars Eckmann
Jul 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jingwen WangSerap Aksoy
Nov 6, 2009·Cellular Microbiology·Maria KaparakisRichard L Ferrero
Dec 19, 2009·PLoS Pathogens·Mathilde GendrinBruno Lemaitre
Jan 19, 2010·Nature Medicine·Thomas B ClarkeJeffrey N Weiser
Mar 29, 2011·BMC Evolutionary Biology·Adriana M MontañoYoko Satta
Aug 1, 2010·Environmental Microbiology·Joshua V TrollMargaret J McFall-Ngai
Oct 25, 2011·Science·Frédéric H LoginAbdelaziz Heddi
Nov 15, 2011·Nature Reviews. Immunology·Julien RoyetRoman Dziarski
Mar 2, 2012·BMC Microbiology·Aurélien VigneronAbdelaziz Heddi
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jingwen Wang, Serap Aksoy
Jul 10, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claudine NeyenBruno Lemaitre
Feb 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Margaret McFall-NgaiJennifer J Wernegreen
Jan 11, 2014·Genome Biology and Evolution·Kelly F OakesonColin Dale
Apr 26, 2014·Science·UNKNOWN International Glossina Genome Initiative

❮ Previous
Next ❯

Citations

Sep 24, 2020·Microorganisms·Nicolas Salcedo-PorrasCarl Lowenberger
Aug 10, 2019·Frontiers in Microbiology·Katrien TrappeniersLinda De Vooght
Aug 11, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Nicole M GerardoKayla S Stoy
Jul 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Justin MaireAbdelaziz Heddi
Aug 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Martin Kaltenpoth
Mar 3, 2020·Frontiers in Microbiology·Jennifer R BratburdHeidi A Horn
Nov 17, 2020·Communicative & Integrative Biology·Justin MaireAbdelaziz Heddi
Mar 11, 2021·Periodontology 2000·Heidi Goodrich-Blair
Jun 3, 2021·International Journal of Molecular Sciences·Julien OrlansPedro Da Silva
Aug 15, 2021·Nature Reviews. Genetics·Julie Perreau, Nancy A Moran

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear translocation
electrophoresis
PCR
Reverse-Transcription

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.