Well defined dye adsorbents for protein purification

Journal of Molecular Recognition : JMR
J Scoble, R K Scopes

Abstract

When activating a matrix with epichlorohydrin, the degree of activation can be controlled by the amount of epichlorohydrin added and the length of time of the reaction. The epoxy group can react with thiols and amines to produce N- and S-linked adsorbents for protein purification. The epoxy-activated gel may also react with ammonia to produce an amino-activated adsorbent. This adsorbent reacts readily with the reactive groups of textile dyes, coupling the dyes to the matrix via a short spacer arm. Conditions can be found where the reaction of the dyes with hydroxyl groups on the matrix is minimized, producing dye-ligand adsorbents with well defined ligand densities. These dye adsorbents can be made with much higher ligand densities than are normally achievable with the conventional coupling via hydroxyl groups on the matrix. The protein-binding behaviour of the highly substituted adsorbents is qualitatively similar to that of the conventional adsorbents but the capacity increases with increase in ligand density. Too high a ligand density is undesirable as it may become difficult to elute the proteins from the adsorbent. The optimum ligand density was found to be between 2 and 10 mumol dye ligand per mL adsorbent, the levels cho...Continue Reading

Citations

Dec 31, 1997·Journal of Chromatography. a·J A Scoble, R K Scopes
Mar 17, 1999·Journal of Molecular Recognition : JMR·J A Scoble, R K Scopes
Aug 17, 2010·Journal of Colloid and Interface Science·Sagheer A OnaiziAnton P J Middelberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Applied and Environmental Microbiology
K A ErlandsonC A Batt
Archives of Biochemistry and Biophysics
G L ELLMAN
© 2021 Meta ULC. All rights reserved