West Nile Virus Capsid Protein Interacts With Biologically Relevant Host Lipid Systems

Frontiers in Cellular and Infection Microbiology
Ana S MartinsNuno C Santos

Abstract

West Nile and dengue viruses are closely related flaviviruses, originating mosquito-borne viral infections for which there are no effective and specific treatments. Their capsid proteins sequence and structure are particularly similar, forming highly superimposable α-helical homodimers. Measuring protein-ligand interactions at the single-molecule level yields detailed information of biological and biomedical relevance. In this work, such an approach was successfully applied on the characterization of the West Nile virus capsid protein interaction with host lipid systems, namely intracellular lipid droplets (an essential step for dengue virus replication) and blood plasma lipoproteins. Dynamic light scattering measurements show that West Nile virus capsid protein binds very low-density lipoproteins, but not low-density lipoproteins, and this interaction is dependent of potassium ions. Zeta potential experiments show that the interaction with lipid droplets is also dependent of potassium ions as well as surface proteins. The forces involved on the binding of the capsid protein with lipid droplets and lipoproteins were determined using atomic force microscopy-based force spectroscopy, proving that these interactions are K+-depende...Continue Reading

References

Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·V AgnelloQ X Zhang
Feb 26, 2000·The Journal of Investigative Dermatology·L J JohnstonN J King
Mar 27, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Eric C M van GorpJos W M van Der Meer
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Robert J Cushley, Mark Okon
May 28, 2003·Journal of Virology·Christopher T JonesRichard J Kuhn
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lixin MaCarol Beth Post
Dec 21, 2004·Nature Reviews. Microbiology·Suchetana MukhopadhyayMichael G Rossmann
Jan 15, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Marco M DominguesNuno C Santos
Sep 9, 2008·Biochimica Et Biophysica Acta·Sven-Olof OlofssonJan Borén
Mar 25, 2009·Tropical Medicine & International Health : TM & IH·Jyoti Chandrashekar Suvarna, Pankaj P Rane
Jun 9, 2009·Antiviral Research·Michael S Diamond
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jamel MankouriMark Harris
Sep 10, 2009·Annual Review of Entomology·William K Reisen
Sep 17, 2009·Biochemical Society Transactions·John McLauchlan
Oct 24, 2009·PLoS Pathogens·Marcelo M SamsaAndrea V Gamarnik
May 11, 2010·Protein and Peptide Letters·Roland Ivanyi-Nagy, Jean-Luc Darlix
May 19, 2010·Biochimica Et Biophysica Acta·Kai WangBing Sun
Jun 2, 2010·Clinics in Laboratory Medicine·Shannan L RossiJared D Evans
Nov 16, 2010·Cell Host & Microbe·Nicholas S Heaton, Glenn Randall
Dec 15, 2010·Trends in Microbiology·Ralf BartenschlagerPatrice André
Mar 4, 2011·Journal of Virology·Pei-Yin LimKristen A Bernard
Apr 6, 2011·PloS One·Filomena A CarvalhoNuno C Santos
Oct 14, 2011·Viruses·Stephanie M LimByron E E Martina
Mar 21, 2012·The Biochemical Journal·Ivo C MartinsAndrea T Da Poian
Mar 30, 2012·PLoS Pathogens·Rushika PereraRichard J Kuhn
May 3, 2012·IUBMB Life·Filomena A Carvalho, Nuno C Santos
Dec 12, 2012·Archives of Biochemistry and Biophysics·Filomena A CarvalhoNuno C Santos
Jun 25, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·André F FaustinoNuno C Santos
Aug 2, 2013·Journal of Dispersion Science and Technology·Vuk Uskoković
Nov 23, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Marco M DominguesNuno C Santos
Apr 22, 2014·International Journal of General Medicine·Timothy J Gray, Cameron E Webb
Nov 21, 2014·ACS Chemical Biology·André F FaustinoIvo C Martins
Jul 15, 2015·Scientific Reports·André F FaustinoNuno C Santos
Dec 30, 2015·Biochimica Et Biophysica Acta·Chao-Wen Wang
Apr 12, 2016·Biochimica Et Biophysica Acta·Ludovico MiglioloOctavio L Franco
May 18, 2016·Nature Nanotechnology·Ana Filipa GuedesNuno C Santos
Oct 30, 2016·Cellular Microbiology·Philippe Roingeard, Rossana C N Melo
May 17, 2017·Biophysics Reviews·Jörg StetefeldTrushar R Patel

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Citations

May 31, 2020·The FEBS Journal·Taniya BhardwajRajanish Giri
Feb 6, 2020·Expert Opinion on Drug Discovery·Alessandro SinigagliaLuisa Barzon
Aug 11, 2020·Current Tropical Medicine Reports·Amy Y VittorSteven T DeKosky
Aug 11, 2019·International Journal of Molecular Sciences·André F FaustinoIvo C Martins
Jan 30, 2021·FEMS Microbiology Reviews·Ebony A MonsonKarla J Helbig
Nov 3, 2020·Frontiers in Molecular Biosciences·Francesca PagliariLuca Tirinato
Mar 16, 2021·Current Opinion in Virology·Thais C Neves-MartinsAndrea T Da Poian
Mar 6, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jade CochardPhilippe Chouteau
Jun 25, 2021·The Journal of Cell Biology·Marta BoschAlbert Pol
Jul 7, 2021·Expert Review of Molecular Diagnostics·Quentin Le BastardEmmanuel Montassier
Sep 30, 2021·Annual Review of Virology·Khyati GirdharEmrah Altindis
Aug 14, 2020·Biochimica Et Biophysica Acta. Biomembranes·Kumar Udit SaumyaRajanish Giri

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Methods Mentioned

BETA
dynamic
atomic force microscopy
circular dichroism
Dynamic light scattering
light scattering
AFM
force
force spectroscopy
force spectroscopy measurements
force measurements

Software Mentioned

JPK

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