Western blot analysis of the antibody response in patients with Listeria monocytogenes meningitis and septicemia

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
J RennebergP Christensen

Abstract

The antibody response in patients with Listeria monocytogenes septicemia and/or meningitis was investigated using Western blot analysis (WBA). Protein antigen preparations were produced from two strains of Listeria monocytogenes, representing serogroup 1 and 4, by sonication and differential centrifugation. IgG antibodies from 8 (50%) of 16 patients with culture verified septicemia and/or meningitis due to Listeria monocytogenes reacted with a 93 kDa antigen from serogroup 1, in contrast to IgG antibodies from only 1 (2%) of 51 controls; these controls represented 21 patients with infections caused by other bacteria and 30 apparently healthy blood donors. Furthermore, IgM antibodies from 3 (19%) of the patients with listeric infections bound to a 106 kDa protein antigen in contrast to none of the controls. In 3 (33%) of 9 patients from whom acute and convalescence serum were available, the patients responded by producing antibodies against new protein antigens. Current methods used in routine serological investigations, i.e. complement fixation and O-agglutination tests, were positive in only 4 (24%) of the 16 patients with listeriosis. The results point to the possibility of designing new immunoassays for detection of septicem...Continue Reading

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Citations

Feb 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J RennebergP Christensen
Nov 1, 1995·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·W H Traub, D Bauer
Feb 13, 2001·Oral Microbiology and Immunology·M L WallengrenU Johnson
Dec 1, 1992·Oral Microbiology and Immunology·L WiderstromK Hamberg
Apr 27, 2004·Oral Microbiology and Immunology·M L Lundin WallengrenD Ericson
Feb 22, 2005·Oral Microbiology and Immunology·M L L WallengrenJ Nordberg
Jan 1, 1997·Critical Reviews in Microbiology·A K Bhunia
Jul 18, 2019·The Journal of Small Animal Practice·S UlrichR Dörfelt
Jan 1, 1995·Clinical and Diagnostic Laboratory Immunology·J J ChristensenA Forsgren

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