PMID: 9171452Nov 1, 1996Paper

Western blot analysis of the reactivity between envelope proteins of hepatitis B viruses from Brazilian carriers and antibodies raised against recombinant hepatitis B vaccines

Acta Virologica
L De CastroS A Gomes

Abstract

A Western blot assay was standardized to evaluate the antigenic reactivity of hepatitis B virus (HBV) strains circulating in Brazilian population with antibodies raised against recombinant hepatitis B (HB) vaccines. In this assay, HBV envelope proteins from infected human blood were detected by antibodies from rabbits immunized with either of two recombinant vaccines. These were Engerix B (Smith Kline Beecham, Belgium) containing exclusively S protein particles and TGP-943 (Takeda Chemical Industries, Japan) containing M protein particles. Forty-seven serum samples, presenting HB surface antigen. (HBsAg) reverse passive haemagglutination assay (RPHA) titers ranging from 1:32 to > or =1:4096 after HBV particles concentration, were tested. Twenty-seven samples were from acute hepatitis cases and 20 were from chronic cases (11 from cirrhotic patients and 9 from asymptomatic carriers). Four HBV serotypes, adw2, adw4, ayw2 and ayw3, were identified in these samples. Infectivity of these sera was evaluated by HBV DNA detection by polymerase chain reaction (PCR). HBV DNA was present in 62% of samples from acute cases and in all samples from chronic cases. Despite the differences between serotypes, genotypes, forms of infection, and in...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.