Western blot immunoassay as a confirmatory test for the presence of anti-Mycoplasma hyopneumoniae antibodies in swine serum

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Mehrdad AmeriWalter H Hsu

Abstract

A Western blot immunoassay (WBI) was developed as a confirmatory test for 2 commercial Mycoplasma hyopneumoniae (Mhyo) ELISAs. The WBI detected at least 5 antigen bands (150, 130, 74, 70, and 30 kDa) from Mhyo whole membrane proteins that were not present in the antigens prepared from M. hyorhinis and M. hyosynoviae. Among discrepant sera from vaccinated pigs (n = 17) and field samples (n = 91) assayed by WBI: 1) 2 of the ELISA-positive samples reacted with all 5 antigens bands; 2) all blocking ELISA-positive samples (n = 53) bound 150-, 130-, 74-, and 70-kD antigen bands; and 3) all indirect ELISA-positive samples (n = 55) bound 150-, 130-, and 30-kD antigens. We conclude that the WBI targeting the top 4 antigen bands is a useful confirmatory test for samples initially screened using the commercial Mhyo ELISAs.

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Feb 5, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mehrdad Ameri-MahabadiWalter H Hsu

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Citations

Jun 12, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Chuan YuAi-Jian Qin
Apr 9, 2008·The Veterinary Journal·Marina SibilaJoaquim Segalés
Aug 17, 2016·Veterinary Microbiology·Andrew C PetersenF Chris Minion
May 18, 2019·Transboundary and Emerging Diseases·Alyssa M BetlachMaria Pieters

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