Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo.

Journal of Clinical Microbiology
Y HayashiM Ohashi

Abstract

Small, round-structured virus (SRSV) was detected in a stool specimen of a patient during an acute gastroenteritis outbreak in Tokyo and was tentatively named SRSV-9. SRSV-9 was purified by sucrose velocity gradient centrifugation after CsCl density gradient centrifugation. The buoyant density of SRSV-9 appeared to be 1.36 g/ml in CsCl. A Western blot (immunoblot) assay using the biotin-avidin system revealed that SRSV-9 was antigenically related to the Hawaii agent but distinct from the Norwalk agent and contained a single major structural protein with a molecular size of 63.0 +/- 0.6 kilodaltons. The prevalence of SRSV-9 infection in Tokyo was surveyed by the Western blot antibody assay by using a crude virus preparation as the antigen. Seroconversion was observed in 56.5% of the patients involved in the outbreaks from which SRSV was detected by electron microscopy.

References

Jul 14, 1977·The New England Journal of Medicine·T A ParrinoN R Blacklow
Oct 6, 1979·The Medical Journal of Australia·A M MurphyR H Millsom
Nov 1, 1987·The Journal of Infectious Diseases·W D CubittS Chiba
Aug 1, 1985·Journal of Clinical Microbiology·G W GaryL J Anderson
Oct 1, 1985·Journal of Medical Virology·J E HerrmannN R Blacklow
Mar 13, 1986·The New England Journal of Medicine·D L MorseG Cukor
May 1, 1986·Journal of Medical Virology·R DolinH P Madore
May 1, 1986·Journal of Virology·H P MadoreR Dolin
Aug 1, 1982·Infection and Immunity·G CukorN R Blacklow
Jun 1, 1984·Microbiological Reviews·G Cukor, N R Blacklow
Jan 1, 1983·Archives of Virology·H TerashimaT Nakao
Mar 1, 1981·Journal of Virology·H B GreenbergR M Chanock

❮ Previous
Next ❯

Citations

Jan 1, 1996·Journal of Medical Virology·K ShinozakiB Simizu
May 11, 2000·The Journal of Infectious Diseases·X JiangD O Matson
Oct 21, 2000·FEMS Microbiology Letters·A M DastjerdiJ C Bridger
Jul 25, 1991·The New England Journal of Medicine·N R Blacklow, H B Greenberg
Oct 1, 1990·Journal of Medical Virology·H P MadoreR Dolin
Jan 1, 1995·Archives of Virology·X JiangM K Estes
Sep 1, 1993·Journal of Medical Virology·M B TaylorW D Cubitt
Jan 1, 1994·Archives of Virology·E T UtagawaS Yamazaki
Feb 1, 1997·The Journal of Veterinary Medical Science·M C San GabrielM Mochizuki
Jan 1, 1996·Archives of Virology·J P LeiteS S Monroe
Jan 9, 2001·Clinical Microbiology Reviews·R L Atmar, M K Estes
Mar 14, 2020·Foods·Lingling Liu, Matthew D Moore
May 11, 2000·The Journal of Infectious Diseases·T AndoR L Fankhauser
Jul 1, 1993·Clinical Microbiology Reviews·C W Hedberg, M T Osterholm
Jun 1, 1990·Journal of Clinical Microbiology·S OkadaM Ohashi
Sep 1, 1990·Baillière's Clinical Gastroenterology·W D Cubitt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.