Western immunoblotting with five Treponema pallidum recombinant antigens for serologic diagnosis of syphilis

Clinical and Diagnostic Laboratory Immunology
V SambriRoberto Cevenini

Abstract

Five immunodominant Treponema pallidum recombinant polypeptides (rTpN47, rTmpA, rTpN37, rTpN17, and rTpN15) were blotted onto strips, and 450 sera (200 from blood donors, 200 from syphilis patients, and 50 potentially cross-reactive) were tested to evaluate the diagnostic performance of recombinant Western blotting (recWB) in comparison with in-house whole-cell lysate antigen-based immunoblotting (wclWB) and T. pallidum hemagglutination (MHA-TP) for the laboratory diagnosis of syphilis. None of the serum specimens from blood donors or from potential cross-reactors gave a positive result when evaluated by recWB, wclWB, or MHA-TP. The evaluation of the immunoglobulin G immune response by recWB in sera from patients with different stages of syphilis showed that rTmpA was the most frequently identified antigen (95%), whereas only 41% of the specimens were reactive to rTpN37. The remaining recombinant polypeptides were recognized as follows: rTpN47, 92.5%; rTpN17, 89.5%; and rTpN15, 67.5%. The agreement between recWB and MHA-TP was 95.0% (100% with sera from patients with latent and late disease), and the concordance between wclWB and MHA-TP was 92.0%. The overall concordance between recWB and wclWB was 97.5% (100% with sera from pa...Continue Reading

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Citations

Oct 30, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M DonatiR Cevenini
Jun 10, 2005·Veterinary Research Communications·A MarangoniR Cevenini
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Oct 8, 2005·Clinical and Diagnostic Laboratory Immunology·Antonella MarangoniRoberto Cevenini
May 24, 2013·Clinical and Vaccine Immunology : CVI·Antonella MarangoniRoberto Cevenini
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