Wet-milling transgenic maize seed for fraction enrichment of recombinant subunit vaccine.

Biotechnology Progress
Lorena MoellerK Wang

Abstract

The production of recombinant proteins in plants continues to be of great interest for prospective large-scale manufacturing of industrial enzymes, nutrition products, and vaccines. This work describes fractionation by wet-milling of transgenic maize expressing the B subunit of the heat-labile enterotoxin of Escherichia coli (LT-B), a potent immunogen and candidate for oral vaccine and vaccine components. The LT-B gene was directed to express in seed by an endosperm specific promoter. Two steeping treatments, traditional steeping (TS, 0.2% SO(2) + 0.5% lactic acid) and water steeping (WS, water only), were evaluated to determine effects on recovery of functional LT-B in wet-milled fractions. The overall recovery of the LT-B protein from WS treatment was 1.5-fold greater than that from TS treatment. In both steeping types, LT-B was distributed similarly among the fractions, resulting in enrichment of functional LT-B in fine fiber, coarse fiber and pericarp fractions by concentration factors of 1.5 to 8 relative to the whole kernels on a per-mass basis. Combined with endosperm-specific expression and secretory pathway targeting, wet-milling enables enrichment of high-value recombinant proteins in low-value fractions, such as the ...Continue Reading

References

Mar 13, 1998·Biotechnology Progress·A R KusnadiZ L Nikolov
May 21, 1999·Advances in Experimental Medicine and Biology·E E HoodJ A Howard
Apr 25, 2000·Journal of Agricultural and Food Chemistry·O D DaileyJ C Mayorga
Mar 21, 2001·Vaccine·S J StreatfieldJ A Howard
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Marija TauschekRoy M Robins-Browne
Dec 14, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Barry J LamphearJohn A Howard
Jan 18, 2003·Vaccine·Stephen J StreatfieldJohn A Howard
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rachel K ChikwambaKan Wang
Oct 29, 2003·Injury·Michael Wagner
Mar 5, 2004·Plant Cell Reports·M E HornJ A Howard
Oct 12, 2004·Vaccine·Carol O TacketStephen Streatfield
Oct 22, 2004·QJM : Monthly Journal of the Association of Physicians·D A Goldstein, J A Thomas
Sep 21, 2005·Bioresource Technology·C I ThompsonM E Tumbleson
Apr 28, 2006·Applied Microbiology and Biotechnology·Om V SinghRakesh K Jain
Oct 13, 2006·Journal of Agricultural and Food Chemistry·Qixin ZhongCharles E Glatz
Jan 9, 2007·Plant Biotechnology Journal·Stephen J Streatfield
Jun 6, 2009·Journal of Experimental Botany·Lorena MoellerKan Wang

❮ Previous
Next ❯

Citations

Nov 25, 2018·International Journal of Molecular Sciences·Michał BurdukiewiczPaweł Mackiewicz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.