WGS-based characterization of the potentially beneficial Enterococcus faecium EFD from a beehive.

Molecular Biology Reports
Svetoslav G DimovTanya Strateva

Abstract

Nowadays, due to their potential application as probiotics for humans or animals, many beneficial lactic acid bacteria have been isolated from different natural environments. These include members of the genus Enterococcus - quite specific due to their ambiguous nature, varying from pathogens to probiotics. In our work we present a whole-genome sequencing (WGS)-based approach for assessing the potential of bacteriocin-producing Enterococcus isolates from beehives to serve as natural preserving agents against bacterial infections associated with honeybees. Potential Enterococcus spp. isolates from pollen granules were tested with the well diffusion assay for bacteriocin activity against Paenibacillus larvae, the causative agent of the American foulbrood disease (AFB). Two of them gave positive results and were determined at species level by 16S rRNA genes sequencing. They were then subjected to WGS using the Illumina HiSeq platform. The resulting raw data reads were processed and further analyzed by using only freely available web-based tools (the Shovill pipeline, QUAST, BAGEL4, ResFinder, VirulenceFinder and PlasmidFinder). The analysis revealed that both of them represent clonally identical isolates of the same strain. This s...Continue Reading

References

Sep 13, 2007·Current Microbiology·Svetoslav G Dimov
Apr 23, 2009·Microbiology·Katie Fisher, Carol Phillips
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Mar 1, 2010·Beneficial Microbes·S G DimovI Ivanova
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Jan 8, 2020·International Journal of Environmental Research and Public Health·Fatin Aina Zulkhairi AminNorhasnida Zawawi

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Datasets Mentioned

BETA
AAD29132.1
CP003351.1

Software Mentioned

Galaxy
BAGEL4
MLST
VirulenceFinder
BLAST
ResFinder3
PlasmidFinder
QUAST
ResFinder

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