Sep 16, 2020

What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder

Cellular and Molecular Neurobiology
Yadi GuanFang Han

Abstract

Studies from postmortem and animal models have revealed altered synapse morphology and function in the brain of posttraumatic stress disorder (PTSD). And the effects of PTSD on dendrites and spines have been reported, however, the effection on axon include microtubule (MT) and synaptic vesicles of presynaptic elements remains unknown. Hippocampus is involved in abnormal memory in PTSD. In the present study, we used the single prolonged stress (SPS) model to mimic PTSD. Quantitative real-time polymerase chain reaction (RT-qPCR) and high-throughput sequencing (GSE153081) were utilized to analyze differentially expressed genes (DEGs) in the hippocampus of control and SPS rats. Immunofluorescence and western blotting were performed to examine change in axon-related proteins. Synaptic function was evaluated by measuring miniature excitatory postsynaptic currents (mEPSCs). RNA-sequencing analysis revealed 230 significantly DEGs between the control and SPS groups. Gene Ontology analysis revealed upregulation in axonemal assembly, MT formation, or movement, but downregulation in axon initial segment and synaptic vesicles fusion in the hippocampus of SPS rats. Increased expression in tau, β-tubulin MAP1B, KIF9, CCDC40, DNAH12 and decrea...Continue Reading

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Mentioned in this Paper

Vesicle-Associated Membrane Protein 2
Gene Ontology
Autopsy
Heat shock proteins
Quantitative Real-Time Polymerase Chain Reaction
Presynaptic Membrane
Synaptotagmin I
Microtubule-associated protein 1B
Protein Expression
Gene Expression

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