What it means to measure a single molecule in a solution by fluorescence fluctuation spectroscopy

Experimental and Molecular Pathology
Z Földes-Papp

Abstract

Traditional methodologies in micro- and nanofluidics measure biological mechanisms as an average of a population of molecules as only their combined effect can be detected. Fluorescence fluctuation spectroscopy methods such as fluorescence correlation spectroscopy (FCS) and two-color fluorescence cross-correlation spectroscopy (FCCS) are used as alternative experimental approaches in ultrasensitive analytics at the single-molecule level. However, what is the measurement time in which one is able to study just one single molecule in solution without immobilizing it? Existing theories are inadequate since they do not predict the meaningful time as a function of the concentration of other molecules of the same kind in bulk solution. This situation produces considerable concern, and experimental hypotheses differ according to which single-molecule detection methods are thought to have greater validity. This subject is clearly at the forefront of research and should be of great interest to experimental medical scientists. As will be seen in this article, it is worthwhile to obtain a correct form of the meaningful-time relationship through theoretical means. The new ideas are comprehensively presented, and this relationship is a new ...Continue Reading

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Citations

Jan 12, 2011·Annual Review of Physical Chemistry·Michelle A Digman, Enrico Gratton
Mar 10, 2007·Experimental and Molecular Pathology·Goro NishimuraMamoru Tamura
Feb 17, 2007·Experimental and Molecular Pathology·Christophe BraetHeinz Peter Nasheuer
May 24, 2007·Methods in Cell Biology·Dylan A Bulseco, David E Wolf
Apr 21, 2007·Nature Protocols·Nancy L Thompson, Bridgett L Steele

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