PMID: 9186948May 1, 1997Paper

What technique should be used for routine detection and quantification of HBV DNA in clinical samples?

Journal of Virological Methods
J M PawlotskyD Dhumeaux

Abstract

Detection of hepatitis B virus (HBV) DNA in serum allows monitoring of HBV replication and assessing responses to antiviral treatment. HBV DNA quantification measures virus replication and can be used as a prognosis indicator of liver disease and an index of response to antiviral drugs. The aim of this study was to compare the performances of three HBV DNA detection and/or quantification techniques for assessing HBV replication. Three hundred unselected sera with a request for HBV DNA detection and quantification were tested with a molecular hybridisation technique without amplification (Digene Hybrid-Capture, Murex Diagnostics Ltd), a signal amplification assay based on branched DNA technology (Quantiplex HBV DNA, Chiron diagnostics), and an 'in-house' qualitative, non quantitative target amplification assay based on the polymerase chain reaction (PCR) with primers located in the S gene of the HBV genome. Hybrid-capture and branched DNA gave concordant results in 278 cases (93%). In the 128 samples positive by both assays, DNA titres in pg/ml were related significantly (r = 0.70, P < 0.0001). but branched DNA titres increased more rapidly than hybrid-capture titres when the amount of HBV DNA in the sample increased. Twenty-two...Continue Reading

References

Jan 1, 1992·Journal of Medical Virology·H M SmithR Williams
Sep 1, 1991·Research in Virology·V BarletJ M Seigneurin
Apr 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B L BakerJ H Hoofnagle
Jun 13, 1991·The New England Journal of Medicine·T J LiangE Ben-Porath
Jan 1, 1990·Journal of Clinical Laboratory Analysis·S KanekoR H Miller
Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J H HoofnagleH Popper
Mar 1, 1985·Annals of Internal Medicine·S C GordonE R Schiff
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C BréchotP Tiollais
Sep 1, 1981·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F BoninoJ Gerin
Nov 27, 1993·Lancet·T L Wright, J Y Lau
Jun 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M RankiP Lehtovaara
Nov 27, 1993·Lancet·J Y Lau, T L Wright

❮ Previous
Next ❯

Citations

Jan 8, 2004·Journal of Hepatology·Jean-Michel Pawlotsky
Jan 6, 2000·Journal of Hepatology·J M Pawlotsky
Jun 9, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·S Badur, A Akgün
Sep 14, 2001·Journal of Viral Hepatitis·G V Papatheodoridis, S J Hadziyannis
Mar 8, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A ErhardtD Häussinger
Oct 17, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·George V PapatheodoridisAthanasios J Archimandritis
Dec 25, 2007·The Korean journal of laboratory medicine·Sang Hyun HwangHeung Bum Oh
Sep 6, 2000·Clinical and Diagnostic Laboratory Immunology·H H KesslerR E Stauber
Mar 7, 2000·Clinical and Diagnostic Laboratory Immunology·H H KesslerR E Stauber
Jun 4, 1999·Journal of Gastroenterology and Hepatology·J H KaoD S Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.