What We See, What We Do Not See, and What We Do Not Want to See in HLA Class I Immunopeptidomes

Proteomics
Michele Mishto

Abstract

The identification of peptides bound to human leukocyte antigen class I (HLA-I) molecules-that is, the HLA-I immunopeptidome-is a useful tool in the hunt for epitopes suitable for vaccinations and immunotherapies. These peptides are mainly generated by proteasomes through peptide hydrolysis and peptide splicing. In this issue, Nicastri and colleagues compared different methods for the elution of HLA class I-associated peptides. It is demonstrated that the choice of HLA-associated peptide enrichment and purification strategy affects peptide yields and creates a bias in detected sequence repertoire. The author carried out this technical brief through the analysis of canonical non-spliced peptides. However, their study left out any analysis of post-translationally spliced peptides, thereby missing an opportunity to shed light on the persistent debate of the frequency of these unconventional peptides.

References

Mar 6, 2004·Science·Nathalie VigneronBenoît J Van den Eynde
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alexandre DaletBenoît J Van den Eynde
Jul 24, 2012·Molecular & Cellular Proteomics : MCP·Michele MishtoPeter Michael Kloetzel
Sep 25, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Celia R BerkersHuib Ovaa
Feb 26, 2016·European Journal of Immunology·Anouk C M PlatteelAlice J A M Sijts
Aug 24, 2017·Trends in Immunology·Michele Mishto, Juliane Liepe
May 4, 2018·Current Opinion in Immunology·Juliane LiepeMichele Mishto
Sep 2, 2018·Molecular & Cellular Proteomics : MCP·Roman MylonasMichal Bassani-Sternberg
Nov 15, 2018·Cancer Immunology Research·Juliane LiepeMichele Mishto
Nov 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Wayne PaesPersephone Borrow

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Citations

Apr 8, 2020·Royal Society Open Science·Damian J RuckDaniel J Lawson
Jan 21, 2021·Biomolecules·Gonca ÇetinFrédéric Ebstein
May 15, 2021·Frontiers in Immunology·Brendan K Reed, John W Kappler
May 23, 2021·Molecular & Cellular Proteomics : MCP·Arie Admon
Dec 24, 2021·The Biochemical Journal·Camila R R BarbosaMichele Mishto

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