Wheat germ agglutinin binding sites in the adult mouse cerebellum: light and electron microscopic studies

The Journal of Comparative Neurology
D A Steindler, N G Cooper

Abstract

The binding properties of derivatized wheat germ agglutinin (WGA) have been examined in fixed tissue sections from the adult mouse cerebellum and also in axonal tracing paradigms following cerebellar injections. The aim of these studies is to begin to distinguish the roles different binding sites may play in generating diverse biological activities which lead to neuronal uptake and axonal transport of lectins or glycoconjugates. Vibratome sections from aldehyde-fixed cerebellum were incubated in N-[acetyl-3H] WGA or WGA conjugated to horseradish peroxidase (WGA-HRP). Sections from this in vitro binding paradigm and those from cerebellar pressure injection cases using those tracers (in vivo binding paradigm) were processed for light microscopic autoradiography, histochemistry, and electron microscopy. Blocking experiments were also performed with various sugar haptens to confirm the binding specificity of these lectin preparations. Light microscopy of lectin binding patterns within the cerebellar cortex has revealed that both derivatized WGA preparations bind most intensely to the molecular layer. Within the deep cerebellar nuclei, binding is unique and produces a punctate delineation of cell bodies and dendrites. Electron micro...Continue Reading

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Citations

Jan 1, 1994·Brain Research. Brain Research Reviews·M R Celio, I Blümcke
Jan 9, 1999·Trends in Neurosciences·M R CelioL Vitellaro-Zuccarello
May 20, 2014·Trends in Molecular Medicine·Kate M Candelario, Dennis A Steindler
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Mar 7, 2021·International Journal of Molecular Sciences·Daniela Carulli, Joost Verhaagen
Apr 1, 2021·Journal of Chemical Neuroanatomy·Akram SadeghiAlireza Ebrahimzadeh-Bideskan
Jan 15, 1988·The Journal of Comparative Neurology·D A SteindlerN G Cooper
Jul 8, 1986·The Journal of Comparative Neurology·N G Cooper, D A Steindler

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